Abstract： Objective： To observe the clinical effect of Chinese herbal fumigation and washing on the basis of blu- ray irradiation and combined B. subtilis and E. faecium granules with multivitamines， live in the treatment of neonates with pathological jaundice. Methods： A total of 120 cases of neonates with pathological jaundice were enrolled and randomly divided into the observation group and the control group，60 cases in each group. Both groups were given irradiation therapy， and the administration of combined B. subtilis and E. faecium granules with multivitamines，live. The observation group was additionally given Chinese herbal fumigation and washing. Both groups were treated for three days. The clinical effect in the two groups was observed. The changes in total bilirubin(TBil)，total bile acid(TBA)，and transcutaneous bilirubin(TCB) in serum in the two groups were monitored. Neonatal behavioral neurological assessment(NBNA) was applied to evaluate neurobehavioral condition of the children. The incidence of adverse reactions during treatment was recorded. Results：After three-day treatment，the total clinical effective rate was 98.33% in the observation group，higher than that of 80.00% in the control group，the difference being significant(P＜0.01). The indexes of TBil and TBA in the two groups were improved when compared with those before treatment，and the two indexes in the observation group were lower than those in the control group， differences being significant(P＜0.05). After one to three days of treatment， the values of TCB in the observation group were lower than those in the control group，differences being significant(P＜0.05). The NBNA scores in the two groups were increased when compared with those before treatment，and the score in the observation group was higher than that in the control group， differences being significant(P＜0.05). During the treatment， no conditions such as rash and fever occurred in the two groups. Conclusion：On the basis of blu-ray irradiation and combined B. subtilis and E. faecium granules with multivitamines，live，the additional therapy of Chinese herbal fumigation and washing in the treatment of neonates with pathological jaundice can further decrease the levels of bilirubin and TBA in serum，reduce the time of blu-ray therapy，and mitigate damage to the liver and impact on neurobehavioral development in children.