Abstract：Objective：To observe the clinical effect of internal and external application of self- made ectopic pregnancy No.2 prescription combined with conservative treatment of routine medicine for ectopic pregnancy(EP). Methods：A total of 120 cases of patients were divided into the control group and the observation group according to the random number table method， 60 cases in each group. Both groups were given two- dose methotrexate(MTX) protocol， and the administration was given according to the descent speed of human chorionic gonadotropin(HCG) until HCG returned to normal or the transfer to operative treatment occurred；mifepristone tablets were applied for five days. The observation group was additionally given internal and external application of self- made ectopic pregnancy No.2 prescription for 14 days. The stopping time of vaginal bleeding， the disappearance time of abdominal pain， the time for HCG to return to normal and the absorption time of pregnancy package were recorded. The clinical effect in the two groups was compared. Six months after treatment， the hysterosalpingography was performed to exam the state of recanalization. Results： After treatment， the cure rate was 91.67% in the observation group，higher than that of 76.67% in the control group，the difference being significant(P＜0.05). The stopping time of vaginal bleeding，the disappearance time of abdominal pain，the time for HCG to return to normal and the absorption time of pregnancy package in the observation group were accelerated when compared with those in the control group(P＜0.01). Six months after treatment， the fallopian tube recanalization rate was 85.00% in the observation group， higher than that of 66.67% in the control group，the difference being significant(P＜0.05). Conclusion：In the short term，the application of internal and external application of self-made ectopic pregnancy No.2 prescription combined with conservative treatment of MTX and mifepristone can shorten the EP course，improve the success rate of conservative treatment. In the long term，it can promote the fallopian tube recanalization，which is conducive to the reproduction of the later period with definite clinical effect.