柚皮素磷脂复合物对氧化损伤的ARPE-19细胞的保护作用
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R285.5

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无锡市卫生和计划生育委员会科研项目(MS201403)


Protective Effect of Naringenin Phospholipid Complex on ARPE-19 Cells with Oxida⁃ tive Damage
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    摘要:

    目的:研究ARPE-19 细胞氧化损伤模型及柚皮素磷脂复合物(NPC) 对氧化损伤的ARPE-19 细胞的保护作用。方法:根据文献最佳制作工艺制备NPC,测定NPC 在水中的溶解度。根据不同浓度的叔丁基氢过氧化物(T-BHP) 对ARPE-19细胞存活率的影响选择最佳的氧化损伤模型。将ARPE-19 细胞分为溶媒组、模型组、柚皮素低剂量组、柚皮素中剂量组、柚皮素组高剂量组、NPC 低剂量组、NPC 中剂量组、NPC 高剂量组。溶媒组用二甲基亚砜(DMSO) 培养;其余各组用200 μmol/L的T-BHP 干预;柚皮素低、中、高剂量组分别用100 μmol/L、200 μmol/L、400 μmol/L 的柚皮素干预;NPC 低、中、高剂量组分别用100 μmol/L、200 μmol/L、400 μmol/L 的NPC 干预。MTT 法检测细胞存活率,流式细胞仪检测细胞凋亡率。结果:与柚皮素比较,柚皮素磷脂混合物、NPC 在水中的溶解度均增大(P<0.01);与柚皮素磷脂混合物比较,NPC 在水中的溶解度增大(P<0.01)。当T-BHP 浓度为200 μmol/L 时,ARPE-19 细胞存活率达半数,是最佳的氧化损伤模型。与溶媒组比较,模型组ARPE-19 细胞存活率明显降低(P<0.01),凋亡率明显升高(P<0.01)。与模型组比较,柚皮素中、高剂量组及NPC 低、中、高剂量组细胞存活率增加(P<0.05),凋亡率下降(P<0.01)。与NPC 低剂量组比较,NPC 中、高剂量组细胞存活率增加(P<0.05),凋亡率下降(P<0.01);柚皮素低、中剂量组细胞凋亡率升高(P<0.01)。与NPC 中剂量组比较,柚皮素低、中、高剂量组和NPC 低剂量组细胞存活率降低(P<0.05),凋亡率升高(P<0.01)。与NPC 高剂量组比较,柚皮素低、中、高剂量组和NPC 低剂量组细胞存活率降低(P<0.05),凋亡率升高(P<0.01)。结论:柚皮素形成磷脂复合物后,水溶性和生物活性提高,对氧化损伤ARPE-19 细胞的保护作用明显增强。

    Abstract:

    Abstract:Objective:To study protective effect of oxidative damage model of ARPE-19 cells and naringenin phospholipid complex(NPC) on ARPE- 19 cells with oxidative damage. Methods: According to the best preparation process recorded on literature, NPC was prepared. The solubility of NPC in water was determined. According to the effect of different concentrations of Tert-butyl hydroperoxlde(T-BHP) on the survival rate of ARPE-19 cells,the best oxidative damage model was selected. ARPE- 19 cells were divided into the solvent group, the model group, the naringenin groups of lowdose, medium-dose and high-dose,the NPC groups of low-dose,medium-dose and high-dose. The solvent group was cultured with dimethyl sulfoxide(DMSO) ; the other group was intervened with 200 μmol/L T-BHP;the naringenin groups of low- dose, medium- dose and high- dose were intervened with 100 μmol/L, 200 μmol/L and 400 μmol/L naringenin respectively;the NPC groups of low- dose,medium- dose group and high- dose were intervened with NPC of 100 μmol/L, 200 μmol/L and 400 μmol/L, respectively. MTT method was used to detect the cell survival rate, and flow cytometry was used to detect the apoptosis rate. Results: Compared with naringenin, the solubility of naringenin phospholipid mixture and NPC in water was increased(P<0.01). Compared with naringenin phospholipid mixture, the solubility of NPC in water was increased(P<0.01). When the concentration of T- BHP was 200 μmol/L,the survival rate of ARPE-19 cells was 50%,which was the best oxidative damage model. Compared with the solvent group,the survival rate of ARPE-19 cells in the model group was significantly decreased(P<0.01),and the apoptosis rate was significantly increased (P<0.01). Compared with the model group,the survival rate in the naringenin groups of medium-dose and high-dose as well as the NPC groups of low- dose, medium- dose and high- dose was increased(P<0.05), and the apoptosis rate was decreased(P<0.01). Compared with the low-dose NPC group,the cell survival rate in the NPC groups of medium-dose and high- dose was increased(P<0.05), and the apoptosis rate was decreased(P<0.01). Compared with the low- dose NPC group,the apoptosis rate in the naringenin groups of low-dose and medium-dose was increased(P<0.01). Compared with the medium-dose NPC group,the survival rate in the naringenin groups of low-dose,medium-dose and high-dose as well as the low- dose NPC group was decreased(P<0.05), and the apoptosis rate was increased(P<0.01). Compared with the high-dose NPC group,the survival rate in the naringenin groups of low-dose,medium-dose and high-dose as well as the low- dose NPC group was decreased(P<0.05), and the apoptosis rate was increased(P<0.01). Conclusion: When the phospholipid complex of naringenin forms, the water solubility and biological activity of naringenin increases, and its protective effect on ARPE-19 cells with oxidative damage is significantly enhanced.

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吉洁,徐新荣,于海涛,周春刚,唐苗苗,唐颖.柚皮素磷脂复合物对氧化损伤的ARPE-19细胞的保护作用[J].新中医,2021,53(6):1-5

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  • 在线发布日期: 2021-03-25
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