Abstract： Objective： To observe the clinical effect of Jianpi Gushen Huayu prescription for diabetic nephropathy. Methods：A total of 108 cases of patients with diabetic nephropathy were selected and divided into the observation group and the control group according to the random number table method，54 cases in each group. The control group was given routine symptomatic treatment， and the observation group was additionally given Jianpi Gushen Huayu prescription based on the treatment of the control group. The clinical effect was compared between the two groups. Before and after treatment，levels of 24- hour urinary micro- albumin excretion rate(UAER)， urinary micro- albumin(mAlb)， urinary connective tissue growth factor(CTGF)，as well as angiogenin(Ang) and its receptor Tie2(Tie- 2) in serum were detected. Before and after treatment， Chinese medicine syndrome scores were counted. Results：The total effective rate was 94.44% in the observation group， higher than that of 79.63% in the control group(P＜0.05). Before treatment， when compared levels of UAER， mAlb， and CTGF in urine between the two groups， there was no significance in the difference(P＞0.05). After treatment， levels of UAER，mAlb，and CTGF in urine in the two groups were decreased when compared with those before treatment，and the three levels in the observation group were lower than those in the control group， differences being significant(P＜0.05). Before treatment，when compared levels of Ang and Tie-2 in serum between the two groups，there was no significance in the difference(P＞0.05). After treatment， levels of Ang and Tie- 2 in serum in the two groups were decreased when compared with those before treatment， and the two levels in the observation group were lower than those in the control group， differences being significant(P＜0.05). Before treatment， when compared Chinese medicine syndrome scores between the two groups，there was no significance in the difference(P＞0.05). After treatment，Chinese medicine syndrome scores in the two groups were decreased when compared with those before treatment，and the scores in the observation group were lower than those in the control group， differences being significant(P＜0.05). Conclusion： For patients with diabetic nephropathy，Jianpi Gushen Huayu prescription can reduce levels of UAER，mAlb，CTGF，as well as Ang and its receptor in serum，relieve clinical symptoms，and improve clinical effect.