Abstract：Objective：To discuss whether the extract of curcuma wenyujin has an effect on proliferation and apoptosis of Parkinson cells through inhibiting microRNA(miRNA)- 103a expression. Methods： SK- N- SH cells were divided into the control group，the model group，the extract of curcuma wenyujin groups of low，medium and high dose(low-dose group， medium-dose group，and high-dose group for short)，the miR-103a group，the miR-NC group，the high-dose+miR-NC group and the high-dose+miR-103a group. The control group was not treated，low-dose，medium-dose，and high-dose groups as well as the model group were induced by 1-methyl-4-phenylpyridiniumion(MPP + )；low-dose，medium-dose， and high-dose groups were given 8，16，and 24 μg/mL extract of curcuma wenyujin respectively. The miR-103a group was transfected with miR- 103a mimic and given MPP + . The miR- NC group was transfected with miR- 103a mimic negative control(miR-NC) and given MPP+. The high-dose+miR-NC group was transfected with miR-NC and given 24 μg/mL extract of curcuma wenyujin. The high-dose+miR-103a group was transfected with miR-103a mimic and given 24 μg/mL extract of curcuma wenyujin. Flow cytometry was used to detect cell cycle and apoptosis in each group. Western blot was used to detect the expression of protein of cysteinyl aspartate specific proteinase3(Caspase3) and nucleus- related antigen Ki- 67. Colorimetric method was applied to measuring levels of malondialdehyde(MDA) and glutathione peroxidase(GSH- Px). Real time fluorescent quantitative PCR(qRT-PCR) was used to detect miR-103a expression. Results：When compared with those in the control group，in the model group，the G0- G1 phase cell proportion，cell apoptosis rate，expression of protein of Caspase3，miR-103a expression ， and MDA content were increased(P ＜ 0.05) ； the S phase cell proportion ， expression of protein of Ki-67，and GSH-Px activity were decreased(P＜0.05). When compared with those in the model group，in the medium- dose and high- dose groups， the G0- G1 phase cell proportion， cell apoptosis rate， expression of protein of Caspase3，miR-103a expression， and MDA content were decreased(P＜0.05)； the S phase cell proportion， expression of protein of Ki- 67，and GSH- Px activity were increased(P＜0.05)，and showed a concentration- dependent manner(P＜ 0.05). When compared with those in the miR- NC group，in the miR- 103a group，the G0- G1 phase cell proportion，cell apoptosis rate，expression of protein of Caspase3，miR-103a expression，and MDA content were increased(P＜0.05)；the S phase cell proportion， expression of protein of K- i67， and GSH- Px activity were decreased(P＜0.05). When compared with those in the high-dose+miR-NC group，in the high-dose+miR- 103a group，the G0-G1 phase cell proportion，cell apoptosis rate，expression of protein of Caspase3，miR-103a expression，and MDA content were increased(P＜0.05)；the S phase cell proportion，expression of protein of K-i67，and GSH-Px activity were decreased(P＜0.05). Conclusion：Extract of curcuma wenyujin can promote proliferation of SK- N- SH cells induced by MPP + ， inhibit their apoptosis， and relieve oxidative stress through down-regulating miR-103a expression.