Abstract：Objective：To observe the clinical effect of Huatan Tongluo tang combined with acupuncture for hemiplegia after stroke and its effect on the levels of albumin(Alb)，glial fibrillary acidic protein(GFAP) and S-100β in serum. Methods：A total of 80 cases of patients with hemiplegia after stroke were selected and divided into the two group，40 cases in each group. Both groups were given routine symptomatic treatment；the control group was additionally treated with acupuncture， and the observation group was additionally treated with Huatan Tongluo tang combined with acupuncture. Both groups were treated for 12 weeks. The scores of Fugl-Meyer Assessment(FMA) and Barthel index as well as the levels of Alb，GFAP and S-100β in serum before and after treatment and the clinical effect in the two groups were compared. Results：The total effective rate was 72.50% in the observation group and 47.50% in the control group，the difference being significant(P＜ 0.05). After treatment，the scores of FMA and Barthel index in the two groups were increased when compared with those before treatment(P＜0.05)，and the scores in the observation group were higher than those in the control group(P＜0.05). After treatment， the levels of Alb， GFAP and S-100β in serum in the two groups were decreased when compared with those before treatment(P＜0.05)，and the levels in the observation group were lower than those in the control group(P＜0.05). Conclusion：The therapy of Huatan Tongluo tang combined with acupuncture for hemiplegia after stroke can improve the clinical effect，the motor function and the self-care ability of patients as well as reduce the levels of Alb，GFAP and S-100β in serum.