Abstract： Objective： To discuss the effect of berberine(Ber) on mature phenotype and phagocytosis of dendritic cells (DC). Methods：DCs were treated with Ber at low，medium and high concentration respectively. After that，fluorescein was used to mark monoclonal antibody CD11c； fluorescein isothiocyanate(FITC)， a kind of fluorescein was used to mark monoclonal antibody major histocompatibility complex Class Ⅰ(MHC Ⅰ)，major histocompatibility complex Class Ⅱ(MHC Ⅱ)， CD40，CD54，CD80，CD86，interleukin-12(IL-12) and tumor necrosis factor-α(TNF-α)；dextran was used to double label DC. Flow cytometry was applied to detect the phenotype，the expression of cytokine and changes in phagocytosis. Results： Compared with that in the control group，the expression of MHC Ⅰ，MHC Ⅱ，CD54，CD80 and CD86 in groups of Ber at various concentrations was increased(P＜0.05)，and the expression of CD40 was decreased(P＜0.05). Compared with that in the low concentration group，in the medium and high concentration groups，the expression of MHC Ⅰ，MHC Ⅱ，CD54 and CD86 was increased(P＜0.05)， and the expression of CD40 was decreased(P＜0.05). Compared with that in the medium concentration group，in the high concentration group，the expression of MHC Ⅰ，MHC Ⅱ，CD54，CD80 and CD86 was significantly increased(P＜0.05)， and the expression of CD40 was decreased(P＜0.05). Compared with that in the control group，the expression level of intracellular cytokines including IL- 12 and TNF- α in groups of Ber at various concentrations was increased(P＜0.05)，and the expression was increased with the increase of Ber concentration(P＜0.05). Compared with that in the control group，in the medium and high concentration groups，the phagocytosis of DC was significantly decreased (P＜0.05). Conclusion：Ber can change the mature phenotype and phagocytosis of DC，which indicates that Ber may affect immune response of the body through DC.