Abstract：Objective：To discuss the effect of total flavones from Bauhinia championii on proliferation and apoptosis of chondrocytes in gonarthritis and its regulatory effect on long non- coding RNAs(LncRNAs) maternally expressed gene 3 (MEG3). Methods： Chondrocytes of rats were separated and cultured； the control group 1 was normally cultured chondrocytes；the control group 2 was cultured for 24 hours in medium with 10 ng/L of interleukin-1β(IL-1β)；the medicine group 1 was cultured for 24 hours in medium with 1 μg/mL of total flavones from Bauhinia championii and 10 ng/L of IL-1β； the medicine group 2 was cultured for 24 hours in medium with 2 μg/mL of total flavones from Bauhinia championii and 10 ng/L of IL-1β；the medicine group 3 was cultured for 24 hours in medium with 3 μg/mL of total flavones from Bauhinia championii and 10 ng/L of IL-1β；in the medicine 3+si-NC group，the chondrocytes were transferred with si-NC，and then treated with 3 μg/mL of total flavones from Bauhinia championii and 10 ng/L of IL-1β for 24 hours；in the medicine 3+ si-MEG3 group，the chondrocytes were transferred with si-MEG3，and then treated with 3 μg/mL of total flavones from Bauhinia championii and 10 ng/L of IL- 1β for 24 hours. The chondrocytes were transferred with pcDNA3.1 and pcDNA3.1- MEG3 respectively，and then cultured with for 24 hours in medium with 10 ng/L of IL-1β，namely the pcDNA3.1 group and the pcDNA3.1- MEG3 group. The cell viability， apoptosis rate and the expressions of interleukin- 6(IL- 6)， tumor necrosis factor-α(TNF-α)，P21，Caspase-3 and BCL-2-associated X protein(Bax) in each group were detected. Results：Compared with those in the control group 1，the cell viability and the MEG3 expression in the control group 2 were decreased(P＜0.05)， and the apoptosis rate and the expressions of P21，Caspase-3，Bax，IL-6 and TNF-α were increased(P＜0.05). Compared with those in the control group 2，the cell viability and the MEG3 expression in the medicine group 1，2 and 3 were increased (P＜0.05)，and the apoptosis rate and the expressions of P21，Caspase-3，Bax，IL-6 and TNF-α were decreased(P＜0.05). Compared with those in the pcDNA3.1 group，the cell viability and the MEG3 expression in the pcDNA3.1-MEG3 group were increased(P＜0.05)，and the apoptosis rate and the expressions of P21，Caspase-3，Bax，IL-6 and TNF-α were decreased (P＜0.05). Compared with those in the medicine 3+si-NC group，the cell viability and the MEG3 expression in the medicine 3+si-MEG3 group were decreased(P＜0.05)，and the apoptosis rate and the expressions of P21，Caspase-3，Bax，IL-6 and TNF-α were increased(P＜0.05). Conclusion：Total flavones from Bauhinia championii may promote the proliferation of chondrocytes in gonarthritis and inhibit the apoptosis and the inflammatory reactions to protect chondrocytes by up-regulating MEG3 expression.