Abstract：The incidence of knee osteoarthritis was analyzed from the changes of channel sinews and ligaments in the knee；the healing of ligament was studied from knee osteoarthritis；the multifactorial effects of ligament- related signaling pathways in the healing of knee osteoarthritis were explored from endogenous and exogenous healing， TGF- β signal transduction pathway，Smad 2/Smad 3 protein，bone morphogenetic protein(BMP)，advantage of acupotomology and other factors. The formation of knee osteoarthritis was closely related to ligament injury and adhesion， and the release of acupotomology not only isolated the invasive growth of the surrounding tissues，but also stimulated the regeneration of the damaged tissues， and that by stimulating and regulating related expression factors， the disease could be prevented and treated，which might be a new idea and provide a new theory for acupotomology treatment of multi-tissue injury.