Abstract： Objective： To observe the effect of Kanglaite injection on immune function in patients with gastrointestinal carcinoma. Methods：A total of 100 cases of patients with gastrointestinal carcinoma were randomly divided into two groups，50 cases in each group. The control group was treated with routine surgery，and the treatment group was additionally treated with Kanglaite injection based on the treatment of the control group. One day before surgery and three weeks after surgery， the levels of T cell subsets including CD3 +，CD4 + and CD8 + and the expression of soluble interleukin 2 receptor(SIL- 2R) in serum were detected. Another 40 healthy people were selected as the healthy control group. Results：The effective rate was 66.00% in the treatment group and 36.00% in the control group，the difference being significant(P＜0.05). Before treatment， the levels of CD3+ and CD4+ in the treatment group and the control group were significantly lower than those in the healthy control group(P＜0.05)，and the levels of CD8+，CD4+/CD8+ and SIL-2R were significantly higher than those in the healthy control group(P＜0.05). After treatment，the levels of CD3 + and CD4 + in the two groups were significantly increased when compared with those before treatment(P＜0.05)， and the levels of CD8 + ， CD4 +/CD8 + and SIL- 2R were decreased when compared with those before treatment(P＜0.05)；the improvement of levels of CD8+，CD4+，CD4+/CD8+ and SIL-2R in the treatment group was significantly better than that in the control group(P＜0.05)，and there was no significant difference being found in the comparison of CD8+ between the two groups(P＞0.05). Conclusion：Based on surgical resection，the application of Kanglaite injection in treating gastrointestinal carcinoma can effectively inhibit the inflammatory responses and improve the immune function of patients.