Abstract：Objective：To observe the clinical effect of Shugan Jianpi tang combined with acupuncture for children with depression and its effect on the levels of neurotransmitters in serum. Methods： A total of 200 cases of children with depression were randomly divided into the control group and the observation group，100 cases in each group. The control group was treated with Shugan Jianpi tang，and the observation group was treated with Shugan Jianpi tang combined with acupuncture. Both groups were treated for 12 weeks. Before and after treatment， the changes in the levels of 5-hydroxytryptamine(5- HT)，dopamine(DA)，norepinephrine(NE) and γ- aminobutyric acid(GABA) in serum as well as the scores of Hamilton Depression Scale(HAMD) in the two groups were observed and compared. The clinical effect in the two groups was evaluated. Results： The cure rate and total effective rate were 30.00% and 93.00% respectively in the observation group and 15.00% and 81.00% respectively in the control group，the difference being significant(P＜0.05). After treatment，the levels of 5-HT，DA，NE and GABA in serum in the two groups were significantly increased when compared with those before treatment(P＜0.05)，and the above levels in the observation group were all higher than those in the control group(P＜0.05). After treatment，HAMD scores in the two groups were significantly decreased when compared with those before treatment(P＜0.05)， and HAMD score in the observation group was lower than that in the control group(P＜0.05). Conclusion： The therapy of Shugan Jianpi tang combined with acupuncture for children with depression has significant curative effect on children with depression， and can improve the levels of neurotransmitters in serum and depression， whose curative effect is better than that of simple Chinese herbal medicine.