Abstract：Objective：To observe the clinical effect of the additional application of directed Chinese medicine penetration and rehabilitation nursing based on conventional western medicine and nursing measures on knee osteoarthritis(KOA). Methods： A total of 150 KOA patients admitted into the department of orthopedics of our hospital were selected into the clinical study， and were divided into the observation group and the control group according to the random number table method， 75 patients in each group. Both groups were given conventional western medicine and nursing， while the observation group was additionally treated with directed Chinese medicine penetration and rehabilitation nursing. Both groups were treated for four weeks. The Visual Analogue Scale(VAS) was used to evaluate knee pain before and after treatment；the Lysholm Knee Function Scale was used to evaluate knee movement function； the Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC) was used to evaluate the severity；and the Activity of Daily Living(ADL) scale was used to evaluate ADL of knee joint. After four weeks of treatment，clinical effect and nursing satisfaction of patients were observed. Results：After treatment，the total effective rate of clinical effect in the observation group and the control group was 88.00% and 72.00% ， respectively， the difference being significant(P＜0.05). The VAS scores in both groups were decreased when compared with those before treatment，and the Lysholm scores were increased when compared with those before treatment，differences being significant(P＜0.05). The VAS score in the observation group was lower than that in the control group，and the Lysholm score was higher than that in the control group，differences being significant(P＜0.05). The WOMAC scores in both groups were decreased when compared with those before treatment， and the ADL scores were increased when compared with those before treatment， differences being significant(P＜0.05). The WOMAC score in the observation group was lower than that in the control group，and the ADL score was higher than that in the control group， differences being significant(P＜0.05). The total nursing satisfaction rate was 94.67% in the observation group，higher than that of 78.67% in the control group， the difference being significant(P＜0.05). Conclusion： The additional application of directed Chinese medicine penetration and rehabilitation nursing measures based on conventional western medicine and nursing measures，has significant therapeutic effect in treating KOA patients，which can effectively reduce the pain degree of knee joint and the severity，improve the knee joint function and ADL，reduce the recurrence of the disease，and improve their nursing satisfaction.