Abstract： Objective： To identify the main natural saccharides in Huangqi Shengmai yin， and to determinate their contents. Methods： The main natural saccharides in Huangqi Shengmai yin were identified by comparing the peak time on high-performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD) with that of standard reference substance. The content determination of monosaccharide was performed by COSMOSIL 5NH2-MS amino column combined with HPLC-ELSD；the mobile phase was acetonitrile-water(80：20)；the flow rate was 1 mL/min；the column temperature was 35 ℃. Results：The main natural saccharide in Huangqi Shengmai yin was fructose. The HPLC-ELSD fructose detection method based on amino column showed good linear relationship in the concentration range of 0.2 to 16 mg/mL， with an average recovery rate of 98.63% and RSD of 1.76% . Conclusion： Huangqi Shengmai yin has relatively high content of fructose which can be detected via HPLC-ELSD method.