Abstract： Objective： To investigate the inhibitory effect of ginsenoside Rg3 regulating long- chain noncoding RNA (LncRNA) CDKN2B- AS1 on gastric cancer cells. Methods： The gastric cancer cell line SGC- 7901 was selected； the gastric cancer cells in the culture medium with ginsenoside Rg3 were identified as the study group，and the gastric cancer cells without adding drugs were identified as the control group. After 72 hours of culture，the expression levels of mRNA including LncRNA CDKN2B-AS1，neural cadherin(N-cadherin)，epithelial cadherin(E-cadherin)，vascular endothelial growth factor(VEGF)，B cell lymphocytoma-2(Bcl-2) and Bcl-2 related X protein(Bax) in the cells were detected by RT-PCR method ； the expression levels of extracellular signal- regulated kinase(ERK1/2) ， ERK1/2 activated by phosphorylation (p-ERK1/2) and matrix metalloproteinase-9(MMP-9) in the cells were detected by Western Blotting；the inhibition rates of cell proliferation in the two groups were detected with MTT kit；cell proliferation levels in the two groups were detected by CCK-8 kit. Results：Compared with those in the control group，the expressions of mRNA including LncRNA CDKN2B-AS1， VEGF，N-cadherin，Bcl-2 mRNA as well as ERK1/2，p-ERK1/2 and MMP-9 in the cells in the study group were decreased (P＜0.05)； the mRNA expressions of Bax and E- cadherin were increased(P＜0.05)； the inhibition rate of cell growth was increased(P＜0.05)；the ability of cell proliferation was decreased(P＜0.05). Conclusion：During the culture of gastric cancer cells， adding ginsenoside Rg3 can effectively inhibit the proliferation of cancer cells and epithelial- mesenchymal transition (EMT)，which provides a reliable theoretical basis for further clinical trials.