Objective：To observe the clinical effect of anti-cancer prescription for advanced non-small cell lung cancer in adjuvant treatment and its influence on tumor markers，apoptosis genes and protein expressions.Methods：Selected 78 cases of patients with advanced non-small cell lung cancer as study subjects，and divided them into the observation group and the control group，39 cases in each group.The control group was given chemotherapy and symptomatic supportive treatment，while the observation group was additionally supplemented with anti-cancer prescription.Compared the clinical effect and the levels of tumor markers，apoptotis genes and protein expressions before and after treatment in the two groups，and recorded adverse reactions.Results：The total effective rate was 92.3%in the observation group，being higher than that of 69.2% in the control group(P＜0.05).After treatment， the levels of carbohydrate antigen 125(CA-125)，carcinoembryonic antigen(CEA)，neuron specific enolase(NSE)and cytokeratin 19 fragment antigen 21-1(CYFRA21-1)in the two groups were decreased when compared with those before treatment(P＜0.05)；the levels of the four tumor markers mentioned above in the observation group were lower than those in the control group(P＜0.05).After treatment，the expression levels of B lymphocytoma/leukemia-2(Bcl-2)gene in the two groups were decreased when compared with those before treatment(P＜0.05)，and the expression levels of gene-associated X protein(Bax)in the B lymphocytoma/leukemia-2 gene were increased when compared with those before treatment(P＜0.05)；the expression level of Bcl-2 gene in the observation group was lower than that in the control group(P＜0.05)，and the expression level of Bax was higher than that in the control group(P＜0.05).The incidence of adverse reactions in the observation group was 12.8%，being lower than that of 41.0%in the control group(P＜0.05).Conclusion：The application of anti-cancer prescription in the adjuvant treatment can improve the curative effect in treating advanced non-small cell lung cancer，and its mechanism may be related to the induction of cancer cell apoptosis.