Objective：To observe the therapeutic effect of the therapy of electroacupuncture on Tianshu(ST25)for rats with ulcerative colitison(UC)and its effect on the TLR4/NF-κB signaling pathway.Methods：Randomly divided 75 Wistar rats into the blank group， the model group， the electroacupuncture group， the sham electroacupuncture group and salazinsulfonapyridine(SASP)group.In addition to the blank group，the UC rat models in other groups were replicated by 2，4，6 trinitrobenzene sulfonic acid/ethanol solution by enema.The electroacupuncture group received the electroacupuncture on Tianshu(ST25)on both sides.The sham electroacupuncture group received the electroacupuncture on the shame Tianshu(ST25)where located on one inch beside it.The SASP group was treated with 10 mL/kg SASP suspension by gavage.After 14-day treatment，evaluated DAI scores，observed the pathology of colon tissues under the light microscope，and detected the contents of interleukin-1β(IL-1β)in serum，tumor necrosis factor-α(TNF-α)and interleukin-10(IL-10)by ELISA method，as well as observed the expression of protein and mRNA in toll-like receptors4(TLR4)，myeloid differentiation primary response 88(MyD88)and nuclear factor kappa-light-chain-enhancer of activated B cells p65(NF-κB p65)in colon tissues in all groups by Western-blot and RT-PCR.Results：Compared with the blank group，the DAI score and contents of IL-1β and TNF-α in serum in the model group were significantly increased， the content of IL-10 was significantly decreased，and the expression of TLR4，MyD88，and NF-κB p65 in colon tissues was significantly increased(P＜0.05).Compared with the model group，the DAI score in the electroacupuncture group and contents of IL-1β and TNF-α in serum were significantly decreased，the content of IL-10 was significantly increased，and the expression of TLR4，MyD88，and NF-κB p65 in colon tissues was significantly decreased(P＜0.05).Compared with the SASP group，the DAI score in the electroacupuncture group was and content of IL-1β was lower and IL-10 was higher；protein expression of TLR4，MyD88，and NF-κB p65 in the colon tissues was lower(P＜0.05).Conclusion：The therapy of electroacupuncture on Tianshu(ST25)can significantly improve the pathological morphology of colon tissues in rats with UC，reduce DAI score and treat UC.Its mechanism is to inhibit the activation of TLR4/NF-κB signaling pathway.