Objective：To observe the effect of Lishui Tongqiao tang on the expression level of vascular endothelial growth factor(VEGF)and the inflammation of middle ear mucosa in rats with acute secretory otitis media.Methods：Divided healthy male SD rats into six groups randomly，namely the normal control group，model group，cefaclor group，Lishui Tongqiao tang of low，middle and high dose groups.In addition to the normal control group，rats in the other groups were given injection of endotoxin in tympanic bullae to establish animal models of rats with acute secretory otitis media.The concentration of Lishui Tongqiao tang in the high，middle and low dose groups was 1.5 g/mL，1 g/mL and 0.5 g/mL respectively，and each rat in these three groups was given 1.05 mL/100 g by gavage.The concentration of cefaclor was 0.01 g/mL in the cefaclor group，and each rat in it was given 1 mL/100 g by gavage.The normal control group and the model group were given the same amount of normal saline and received continuous medication for seven days.After intervention，the expression level of VEGF in the middle ear mucosa of each group was observed，and the changes in the mucosal lesions of otitis media were examined.Results：Compared with the normal control group，the mucosa thickness of the middle ear drum，the neutrophil count in the middle ear mucosa，and the expression of VEGF in the middle ear mucosa in the model group were remarkably increased，differences being significant(P ＜ 0.05).Compared with the model group，the mucosa thickness of the middle ear drum，the neutrophil count in the middle ear mucosa，and the expression of VEGF in the middle ear mucosa in Lishui Tongqiao tang of low，middle and high dose groups were remarkably decreased，differences being significant(P＜0.05).There was no significant difference being found in the comparison of the above three items among the Lishui Tongqiao tang groups of each dose and the cefaclor group(P＞0.05).Conclusion：Lishui Tongqiao tang can inhibit the expression of VEGF in the middle ear mucosa and reduce the middle ear mucosal edema caused by endotoxin，thus inhibiting the middle ear inflammatory responses.