血塞通注射液及NEPl-40对脑梗死大鼠大脑皮层及血清炎症因子表达的影响
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Xuesaitong Injection and NEP1-40 Has Effect on Cerebral Cortex and Expression of Serum Inflammatory Factors in Rats with Cerebral Infarction
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    摘要:

    目的:分析血塞通注射液及Nogo胞外肽端残基1-40(NEP1-40)对SD大鼠脑梗死大脑皮层及血清中促炎因子与抑炎因子表达的影响。方法:25只雄性健康SD大鼠,依据随机数字表法分成模型组、NEP1-40组、血塞通组、假手术组及NEP1-40联合血塞通组(P+N组),每组5只。依据改良Longa线栓栓塞法建立脑梗死模型,各给药组分别给予相应药物,干预28天后处死大鼠,观察大鼠术后体质量和神经功能评分改变状况及血清、脑组织内白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)、白细胞介素-10(IL-10)的水平。结果:与假手术组比较,模型组大鼠术后7、14、28天体质量明显下降;神经功能评分明显上升;血清TNF-α、TGF-β1含量上升,IL-10含量下降;脑组织TNF-α、IL-1β、TGF-β1蛋白表达上升,IL-10蛋白表达下降;差异均有统计学意义(P<0.05)。与模型组比较,NEP1-40组、血塞通组及P+N组大鼠术后7、14、28天体质量显著上升,神经功能评分明显下降,血清TNF-α含量下降,IL-10含量上升;P+N组大鼠血清IL-β1含量下降;血塞通组及P+N组大鼠脑组织TNF-α、IL-1β蛋白表达下降,TGF-β1、IL-10蛋白表达上升;NEP1-40组大鼠脑组织TNF-α、IL-1β蛋白表达下降,IL-10蛋白表达上升;差异均有统计学意义(P<0.05)。结论:血塞通注射液及NEP1-40通过调整促炎、抑炎因子表达来加快脑梗死大鼠神经功能恢复。

    Abstract:

    Objective:To analyze the effect of Xuesaitong injection and Nogo extracellular peptide residue 1-40(NEP1-40)on cerebral cortex and expression of serum pro-inflammatory factors and anti-inflammatory factors in SD rats with cerebral infarction.Methods:Selected 25 healthy male SD rats,and divided them into the model group,NEP1-40 group,Xuesaitong group,the sham operation group and NEP1-40 combined with Xuesaitong group(P+N group)according to the random number table method,5 SD rats in each group.Established a model of cerebral infarction according to the therapy of modified suture and embolization of Longa.Each group was given corresponding drugs.Killed the rats on the 28thday after intervention.Observed the changes of body mass and scores of neurological function of rats,and observed the level and expression of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),transforming growth factor-β1(TGF-β1)and interleukin-10(IL-10)in serum and brain tissues.Results:When compared with those in the sham operation group,the body mass of rats on the 7th,14th,and 28thday after operation in the model group was obviously decreased,scores of neurological function were obviously increased,the content of serum TNF-α and TGF-β1 was increased,while the content of IL-10 was decreased;the protein expression of TNF-α,IL-1β and TGF-β1 in brain tissues was increased,while the protein expression of IL-10 was decreased,differences were significant(P < 0.05).When compared with that in the model group,the body mass on the 7th,14th,and 28thday after operation in the rats of NEP1-40 group,Xuesaitong group and P+N group was obviously increased,scores of neurological function were obviously decreased,the content of serum TNF-α was decreased,while the content of IL-10 was increased(P< 0.05).When compared with that in the model group,the content of serum IL-β1 in rats of P+N group was decreased;the protein expression of TNF-α and IL-1β in brain tissues of rats in Xuesaitong group and P+N group was decreased,while the protein expression of TGF-β1 and IL-10 was increased;the protein expression of TNF-α and IL-1β in brain tissues of rats in NEP1-40 group was decreased,while the protein expression of IL-10 was increased;differences were significant(P < 0.05).Conclusion:Xuesaitong injection and NEP1-40 can accelerate the recovery of neurological function in ratswith cerebral infarction by adjusting the expression of pro-inflammatory and anti-inflammatory factors.

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何慧君,刘志娟.血塞通注射液及NEPl-40对脑梗死大鼠大脑皮层及血清炎症因子表达的影响[J].新中医,2018,50(6):10-13

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  • 在线发布日期: 2018-06-05
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