Objective：To discuss the effect of Yanghuo Baji oral liquid on sex hormone，sperm density and sperm activate rate of the model of infertile rats with kidney-yang deficiency.Methods：Divided rats into the blank group，the model group，the low-dose Chinese medicine group and the high-dose Chinese medicine group.The model group，low-dose Chinese medicine group and high-dose Chinese medicine group were administered with adenine by gavage for 30 days to establish a model of infertile rats with kidney-yang deficiency，while the blank group was administered with normal saline by gavage.Thirty days later， each group of rats were sacrificed， and detected such indexes as follicle-stimulating hormone(FSH)，teststerone(T)，sperm density and sperm activate rate.Results：There was no significant difference in the body mass of rats in each group on the sixth day(P＜0.05).Since the day 12，comparing with the blank group，the body mass of rats in the model group was significantly decreased，difference being significant(P＜0.05)；comparing with the blank group，the body mass of rats in the low-dose Chinese medicine group and the high-dose Chinese medicine group was increased，differences being significant(P＜0.05).Comparing with the blank group，sperm density，sperm activate rate and the concentration of FSH and T of rats in the model group were significantly decreased，differences being significant(P＜0.05).Comparing with the model group，sperm density，sperm activate rate and the concentration of FSH and T of rats in the low-dose Chinese medicine group and the high-dose Chinese medicine group were significantly increased，differences being significant(P＜0.05).Sperm density，sperm activate rate and the concentration of FSH and T of rats in the high-dose Chinese medicinegroup were higher than those in the low-dose Chinese medicine group，differences being significant(P＜0.05).Conclusion：Yanghuo Baji oral liquid can improve the spermatogenic function and the adjustment of sex hormone in the model of infertile rats with kidney-yang deficiency，and its effect is associated with the progressive increase of dosage.