新麦纤散对溃疡性结肠炎大鼠结肠组织ATF-4/C HOP表达的影响
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R574.64

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浙江省科技计划项目(2016C33085)


New Maixian Powder Has Effect on Expression of ATF-4/CHOP in Colon Tissues of Rats with Ulcerative Colitis
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    摘要:

    目的:观察新麦纤散对溃疡性结肠炎(Ulcerative colitis,UC)大鼠结肠组织活化转录因子-4(Activating transcription factor-4,ATF-4)、C/EBP同源蛋白(C/EBP homologous protein,CHOP) 表达的影响,探究其治疗UC的作用机制。方法:将60只SD大鼠随机分成正常组、模型组、美沙拉嗪组、新麦纤散低、中、高剂量组,每组10只,5%DSS用以诱导UC模型。美沙拉嗪组灌胃美沙拉嗪缓释颗粒混悬液0.42 g/(kg·d),新麦纤散低、中、高剂量组分别灌胃新麦纤散混悬液1.5、3、6 g/(kg·d),其余组大鼠予等体积生理盐水灌胃,连续治疗2周。观察大鼠一般情况,评估病理组织学损伤,采用Western Blot和RT-PCR法分别检测结肠组织ATF-4、CHOP蛋白和mRNA的表达。结果:与正常组比较,模型组大鼠结肠病理损伤评分显著升高,结肠组织ATF-4、CHOP蛋白和mRNA表达增加,差异均有统计学意义(P<0.05)。与模型组比较,美沙拉嗪组、新麦纤散低、中、高剂量组大鼠结肠病理损伤评分显著降低,结肠组织ATF-4、CHOP mRNA表达减少;美沙拉嗪组和新麦纤散中、高剂量组ATF-4、CHOP蛋白表达减少;差异均有统计学意义(P<0.05)。结论:新麦纤散对UC大鼠有较好的疗效,抑制结肠组织ATF-4、CHOP蛋白和mRNA的表达,减少细胞凋亡可能是其作用机制之一。

    Abstract:

    Objective:To observe the effect of new Maixian powder on the expression of activating transcription factor-4(ATF-4),C/EBP homologous protein(CHOP)in colon tissues of rats with ulcerative colitis,and investigate its mechanism in treating UC.Methods:Divided 60 SD rats randomly into the normal group,the model group,mesalazine group and new Maixian powder groups of low,middle and high dose,10 rats in each group.UC model was induced by 5%dextran sulfate sodium(DSS),mesalazine group was given 0.42 g/(kg·d)of mesalazine granules suspension by gavage,while the new Maixian powder groups of low,middle and high dose were respectively given 1.5,3 and 6 g/(kg·d)of new Maixian powder suspension by gavage.Rats in other groups were given saline of the same volume by gavage.Each group continuously received treatment for two weeks.Observed the general situation of rats,evaluated histopathological damage and adopted Western Blot method and RT-PCR method to respectively detect the expression of ATF-4,CHOP protein and mRNA in colon tissues.Results:Compared with those in the normal group,scores of pathological damage of rats in the model group were obviously increased and the expression of ATF-4,CHOP protein and mRNA in colon tissues was increased,differences being significant(P<0.05).Compared with those in the model group,scores of pathological damage of rats in the mesalazine group and the new Maixian powder groups of low,middle and high dose were obviously decreased,and the expression of ATF-4 and CHOP mRNA in colon tissues was decreased;the expression of ATF-4 and CHOP protein in the mesalazinegroup and the new Maixian powder groups of middle and high dose was decreased;differences were significant(P<0.05).Conclusion:New Maixian powder has significant effect in treating UC rats,which can inhibit the expression of ATF-4,CHOP protein and mRNA in colon tissues and one of its mechanisms may be reduce cell apoptosis.

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付敏军,张君,潘珍珍,郑红斌.新麦纤散对溃疡性结肠炎大鼠结肠组织ATF-4/C HOP表达的影响[J].新中医,2018,50(5):1-5

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  • 在线发布日期: 2018-05-03
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