Objective：To discuss the effect of medicated serum of Duhuo Jisheng tang on proliferation and apoptosis of fibroblast-like synoviocytes(FLS)in rats with adjuvant-induced arthritis.Methods：SD rats were applied to produce medicated serum of Duhuo Jisheng tang.Complete Freund's adjuvant was used to induce adjuvant-induced arthritis in rats，and then primary FLS were separated and extracted.FLS were divided into six groups：blank group，model group，methotrexate group，as well as Duhuo Jisheng tang groups of low，middle，and high dose，with five replications in each group.Each group was given corresponding medication respectively.The effect of Duhuo Jisheng tang on cell proliferation rate was detected by cell counting kit 8(CCK8)and expression changes of the levels of cell Bax，Bcl-2 and caspase3 mRNA as well as protein were respectively detected by fluorescence quantitative PCR and ELISA.Results：There was no obvious change in the cells of the blank group at each time point.The proliferation inhibition rate of cells in the model group was increased as the time prolonged(P＜0.05).The proliferation inhibition rate of cells in the methotrexate group and Duhuo Jisheng tang groups of low，middle，and high dose was decreased as the intervention time prolonged(P ＜ 0.05).Compared with the blank group，the proliferation inhibition rate of cells at each time point was increased in the model group(P＜0.05).Compared with the model group，the proliferation inhibition rate of cells at each time point was decreased in the methotrexate group and Duhuo Jisheng tang groups of each dose(P ＜ 0.05)，and especially the decrease of rate in Duhuo Jisheng tang group of high dose was more obvious.Compared with the blank group，levels of Bax，caspase3 mRNA and protein in the model group were decreased，while levels of Bcl-2 mRNA and protein were increased(P ＜ 0.05，P ＜ 0.01).Compared with the model group，levels of Bax，caspase3 mRNA and protein in methotrexate group and Duhuo Jisheng tang groups of middle and high dose were increased，while levels of Bcl-2 mRNA and protein were decreased(P ＜ 0.05，P ＜ 0.01)，and especially the effect in Duhuo Jisheng tang group of high dose was more obvious.Conclusion：Duhuo Jisheng tang can inhibit proliferation of FLS in rats with adjuvant arthritis.The mechanism is related to down-regulation of the expression of anti-apoptotic protein Bcl-2 and up-regulation of the expression of pro-apoptotic protein Bax and caspase3.