十一味益肾降糖方对2型糖尿病模型大鼠血糖及肾组织Wnt/β-catenin信号通路表达的影响
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Effects of Shiyiwei Yishen Jiangtang Prescription on Expression of Blood Glucose and Wnt/β-catenin Signal Pathway in Kidney Tissue of Model Rats with Type 2 Diabetes
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    摘要:

    目的:探讨十一味益肾降糖方对2型糖尿病(NIDDM)大鼠的降血糖作用以及对肾脏Wnt/β-catenin信号通路表达的影响。方法:采用高糖高脂饲养联合注射链脲佐菌素(STZ)的方法制备2型糖尿病模型大鼠。60只SD大鼠随机均分为6组,即正常对照组、模型组、达格列净组和十一味益肾降糖方低、中、高剂量组,每组10只。达格列净组每天灌胃达格列净片1 mg/(kg·d),十一味益肾降糖方低、中、高剂量组分别灌胃十一味益肾降糖方水煎液2、4、16 g/(kg·d),正常对照组和模型组灌胃等量的生理盐水,每天给药1次,连续给药7周。测定大鼠空腹血糖(FBG)水平、糖化血红蛋白(HbA1c)水平和空腹胰岛素(FINS)水平,并计算胰岛素抵抗指数(IRI)。采用Western blot法检测Wnt4、β-catenin蛋白的表达。结果:与正常对照组比较,模型组大鼠FBG、HbA1c、FINS水平升高,IRI升高,Wnt4、β-catenin蛋白的表达量均明显增多,差异均有统计学意义(P<0.05)。给药后,与模型组比较,达格列净组、十一味益肾降糖方中、高剂量组大鼠FBG、HbA1c、FINS水平显著降低,IRI显著降低,Wnt4、β-catenin蛋白的表达量均明显减少,差异均有统计学意义(P<0.05)。与给药前比较,达格列净组、十一味益肾降糖方高剂量组大鼠FBG水平显著降低,差异均有统计学意义(P<0.05)。与给药前比较,达格列净组、十一味益肾降糖方中、高剂量组大鼠HbA1c、FINS水平显著降低,IRI显著降低,差异均有统计学意义(P<0.05)。结论:十一味益肾降糖方可有效控制2型糖尿病大鼠的空腹血糖水平,调节糖代谢,能够抑制糖尿病大鼠肾脏Wnt/β-catenin信号通路的高表达。

    Abstract:

    Objective:To discuss the hypoglycemic effect of Shiyiwei Yishen Jiangtang prescription on rats with type 2 diabetes(NIDDM)and its effect on expression of Wnt/β-catenin signal pathway in kidney.Methods: High-glucose and high-fat diet combined with injection of streptozotocin(STZ)was applied to establish NIDDM model rats.Divided 60 SD rats into 6 groups randomly,namely the normal control group,model group,dapagliflozin group,Shiyiwei Yishen Jiangtang prescription of low,middle and high dose groups,10 rats in each group.Dapagliflozin group was given dapagliflozin tablets 1 mg/(kg·d)by gavage,Shiyiwei Yishen Jiangtang prescription of low,middle and high dose groups were given Shiyiwei Yishen Jiangtang prescription of 2,4,16 g/(kg·d)respectively by gavage,and the normal control group and model group were given equivalent normal saline by gavage,once a day,for 7 weeks successively.Measured the levels of fasting blood glucose(FBG),glycosylated hemoglobin(HbA1c)and fasting insulin(FINS)of rats,and calculated insulin resistance index(IRI).Western blot was induced to detect the expression of Wnt4 and β-catenin protein.Results: Comparing with those in the normal control group,the levels of FBG、HbA1c and FINS were increased,IRI was increased and the expression of Wnt4 and β-catenin protein was both significantly increased in the model group,differences being significant(P< 0.05).After administration,comparing with those in the model group,the levels of FBG、HbA1c and FINS were distinctively decreased,IRI was significantly reduced and the expression of Wnt4 and β-catenin protein was both evidently decreased in dapagliflozin group and Shiyiwei Yishen Jiangtang prescription of middle and high dose groups,differences being significant(P<0.05).Comparing with those before administration,the levels of FBG were distinctively decreased in dapagliflozin group and high-dose Shiyiwei Yishen Jiangtang prescription group,differences being significant(P<0.05).Comparing with those before administration,the levels of HbA1c and FINS were distinctively decreased,IRI was significantly reduced in dapagliflozin group and Shiyiwei Yishen Jiangtang prescription of middle and high dose groups,differences being significant(P< 0.05).Conclusion:Shiyiwei Yishen Jiangtang prescription can effectively control the levels of FBG in rats with type 2 diabetes,adjust glucose metabolism,and inhabit the high expression of Wnt/β-catenin signal pathway in the kidney of NIDDM rats.

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杨娜,刘丰林.十一味益肾降糖方对2型糖尿病模型大鼠血糖及肾组织Wnt/β-catenin信号通路表达的影响[J].新中医,2018,50(3):14-17

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  • 在线发布日期: 2018-03-04
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