肾炎消白颗粒对肾炎模型大鼠Nephrin蛋白表达的影响
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

R692.3

基金项目:


Shenyan Xiaobai Granules Has Effect on Nephrin Protein Expression of Nephritis Models of Rats
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:观察肾炎消白(SYXB)颗粒对肾炎模型大鼠肾脏组织中Nephrin蛋白表达的影响,探讨其作用机理。方法:100只Wistar雄性大鼠随机分为空白组、模型组、洛汀新组、中药低剂量组、中药高剂量组,每组20只。采用一次性尾静脉注射阿霉素制造肾炎蛋白尿大鼠模型。空白组、模型组灌胃蒸馏水2 mL/d,洛汀新组灌胃洛汀新0.90 mg/kg,中药高、低剂量组分别灌胃SYXB颗粒3.60、1.80 g/kg,每天1次,1周连续给药6次,共给药7周。收集大鼠随机尿液,检测尿蛋白排泄量。3周末、5周末分别取3只大鼠,取右肾,7周末所有剩余大鼠取右肾,免疫组化检测大鼠肾组织Nephrin蛋白表达情况,HE染色观察肾脏病理组织学变化。结果:与空白组比较,模型组大鼠各时间点尿白蛋白/尿肌酐比值显著升高,肾组织Nephrin蛋白表达显著降低,差异均有统计学意义(P<0.05,P<0.01)。与模型组比较,洛汀新组大鼠各时间点尿白蛋白/尿肌酐比值显著降低,肾组织Nephrin蛋白表达显著升高;中药高剂量组大鼠在第3、4、5、6、7周尿白蛋白/尿肌酐比值显著降低,各时间点肾组织Nephrin蛋白表达显著升高,差异均有统计学意义(P<0.05)。与洛汀新组比较,中药高剂量组大鼠在第5、6、7周尿白蛋白/尿肌酐比值较低,差异均有统计学意义(P<0.05)。与第3周末比较,洛汀新组、中药高、低剂量组大鼠各时间点肾组织Nephrin蛋白表达显著升高,差异均有统计学意义(P<0.05)。结论SYXB颗粒可能通过上调阿霉素肾病大鼠肾组织中Nephrin蛋白表达,从多部位修复损伤的足细胞。在阿霉素肾病大鼠蛋白尿的治疗中,SYXB颗粒显著优于洛汀新,SYXB颗粒高剂量组优于低剂量组。

    Abstract:

    To observe the effect of Shenyan Xiaobai(SYXB)granules on Nephrin protein expression of nephritis models of rats in renal tissue,and investigate its mechanism.Methods:Divided 100 Wistar male rats into the blank group,the model group,Lotensin group,Chinese medicine low-dose group,and Chinese medicine high-dose group randomly,20 rats in each group.Except the blank group,the other groups were given adriamycin by means of disposable tail vein injection to establish rat models of nephritis proteinuria.The blank group and the model group were given 2 mL/d of distilled water by gavage,and the Lotensin group was given 0.90 mg/kg of Lotensin by gavage,while the Chinese medicine low-dose group and the Chinese medicine high-dose group were respectively given 3.60 g/kg and 1.80 g/kg of SYXB granules,once a day,6 times a week.The administration lasted for 7 weeks in total.Collected random urine of rats,and detected excretion of urine protein.Three rats and their right kidney were respectively collected at the end of 3rdweek and 5thweek,and the right kidney of the rest rats were all collected at the end of 7thweek.Detected the Nephrin protein expression of kidney tissue of rats by using immunohistochemistry,and observed histopathological changes of kidney by using HE staining.Results:Comparing with that of the blank group,urine albumin/urinary creatinine ratio of rats in each time point in the model group was significantly increased,while the Nephrin protein expression of kidney tissue was obviously decreased,differences being significant(P<0.05,P<0.01).Comparing with that of the model group,urine albumin/urinary creatinine ratio of rats in each time point in the Lotensin group was significantly decreased,while the Nephrin protein expression of kidney tissue was evidently increased;urine albumin/urinary creatinine ratio of rats at 3th,4th,5th,6thand 7thweek in the Chinese medicine high-dose group was significantly decreased,while the Nephrin protein expression of kidney tissue in each time point was evidently increased,differences being significant(P<0.05).Comparing with that of the Lotensin group,urine albumin/urinary creatinine ratio of rats at 5th, 6thand 7thweek in the Chinese medicine high-dose group was lower, differences being significant(P<0.05).Comparing with that at the end of 3rdweek,Nephrin protein expression of kidney tissue in each time point in the Lotensin group,the Chinese medicine high-dose and the Chinese medicine low-dose group was significantly increased,differences being significant(P < 0.05).Conclusion:SYXB granules can repair damaged podocytes from multiple locations by up-regulating the expression of Nephrin protein expression of kidney tissue of rat with Adriamycin nephropathy.In the treatment of proteinuria of rat with Adriamycin nephropathy,SYXB granules was significantly better than Lotensin,and the high-dose group of SYXB granules was better than the low-dose one.

    参考文献
    相似文献
    引证文献
引用本文

曹尚美,黄雯静,王立范.肾炎消白颗粒对肾炎模型大鼠Nephrin蛋白表达的影响[J].新中医,2018,50(3):9-13

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2018-03-04
  • 出版日期: