ObjectiveTo evaluate the anti-inflammatory and analgesic effect of electroacupuncture with different parameter combinations，and to select the optimal parameter combination of electroacupuncture in the treatment of inflammatory pain.MethodsDivided 30 male SD rats into blank control group(group N)，the model group(group M)，2 Hz group，100 Hz group and 2/100 Hz group，6 rats in each group.Injected complete Freund's adjuvant(CFA)into plantars of right hind legs of SD rats to establish the rat model of inflammatory pain.Intervened Zusanli and Kunlun on affected sides in rats with electroacupuncture.Before and 24 h after the establishment of model and 1st，3rd，5th，7th，and 10thdays of treatment，detected paw withdrawal thresholds(PWTs)of right hind legs of rats.Adopted enzyme linked immunosorbent assay to detectthe content of inflammatory factors such as interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α).ResultsAt 24 h after the establishment of model，except group N，PWTs of rats in other groups were significantly decreased(P ＜ 0.01)，indicating the success of model establishment.Comparing with those in group M，after intervention with electroacupuncture stimulation，PWTs of electroacupuncture groups at each time point were evidently increased(P＜0.01).At the three time points of the 1st，3rdand 5thday after intervention，detected PWTs of electroacupuncture groups，there being no significance in differences(P＞0.05).On the 7thday after intervention，PWTs in 2/100 Hz group were higher than those in 2 Hz group and 100 Hz group(P＜0.01).On the 9thday after intervention，PWTs in 2/100 Hz group and 100 Hz group were higher than those in 2 Hz group(P＜0.05，P＜0.01).On the 10thday after intervention，PWTs in 2/100 Hz group were higher than those in 2 Hz group(P＜0.01).Comparing with that in group N，the content of IL-1βand TNF-α in group M was significantly increased(P＜0.01).Comparing with that in group M，IL-1βcontent was evidently decreased in 2 Hz group，100 Hz group and 2/100 Hz group(P＜0.05，P＜0.01)；TNF-α content in 100 Hz group and 2/100 Hz group was significantly decreased(P＜0.01).Comparing with that in 2 Hz group，TNF-α content in 100 Hz group and 2/100 Hz group was significantly decreased(P＜0.05，P ＜ 0.01).ConclusionElectroacupuncture with different parameter combinations all have anti-inflammatory and analgesic effect but the analgesic effect can be different.Electroacupuncture with parameter of 2(50 μs)/100 HZ(200 μs)is optimal for the intervention of inflammatory pain.