Abstract： Objective： To observe the clinical effect of yin- yang balance penetration combined with rehabilitation training for upper limb spasticity after stroke. Methods： A total of 84 cases of patients with upper limb spasticity after stroke were selected and divided into the control group and the observation group according to the random number table method， with 42 cases in each group. Both groups were given targeted therapy of western medicine； the control group was additionally treated with rehabilitation training， and the observation group was additionally treated with yin- yang balance penetration based on the treatment of the control group. Both groups were treated for 2 months. Before and after treatment，the degree of upper limb spasticity was evaluated by Ashworth scores，the upper extremity motor function was evaluated by Simplified Fugl-Meyer Assessment (SF-MA)，and the neurological function was evaluated by National Institutes of Health Stroke Scale (NIHSS). Results：After treatment，the Ashworth grading in the two groups were improved when compared with those before treatment (P＜0.05)， and the Ashworth grading in the observation group was better than that in the control group (P＜0.05). After treatment，the NIHSS scores in the two groups were decreased when compared with those before treatment (P＜0.05)， and the SF- MA scores were increased when compared with those before treatment (P ＜ 0.05) ； the SF-MA score in the observation group was higher than that in the control group (P＜0.05)，and there was no significant difference in NIHSS score between the two groups (P＞0.05). Conclusion：Yin-yang balance penetration combined with rehabilitation training in treating upper limb spasticity after stroke， can effectively reduce the symptom of upper limb spasticity of patients and improve their upper extremity motor function.