Abstract: Objective: To observe the clinical effect of Jianpi Tongqiao prescription for chronic secretory otitis media (CSOM) and its effect on auditory function. Methods:A total of 94 cases of CSOM patients were randomly divided into the control group and the observation group, 47 cases in each group. The control group was given the injection of dexamethasone through eustachian tube under endoscope,and the observation group was additionally given Jianpi Tongqiao prescription based on the treatment of the control group. The Chinese medicine syndrome scores, the thresholds of bone conduction at various frequencies including 1 kHz, 2 kHz, 4 kHz and 8 kHz, and the levels of aquaporin- 1(AQP- 1) and aquaporin- 4(AQP- 4) in serum before and after treatment were observed in the two groups; the clinical effect and the recurrence rate were compared between the two groups. Results: The total effective rate was 87.23% in the observation group and 70.21% in the control group, the difference being significant(P<0.05). After treatment, the Chinese medicine syndrome scores including deafness and lusterless complexion as well as thin and unformed stool in the two groups were lower than those in the same group before treatment(P<0.05),and each score in the observation group was lower than that in the control group(P<0.05). After treatment,the thresholds of bone conduction at 1 kHz,2 kHz,4 kHz and 8kHz in the two groups were decreased when compared with those before treatment(P<0.05), and the above each index in the observation group was lower than that in the control group(P<0.05). After treatment,the levels of AQP-1 and AQP-4 in the two groups were higher than those in the same group before treatment(P<0.05),and the two levels in the observation group were higher than those in the control group(P<0.05). The recurrence rate was 12.20% in the observation group and 45.45% in the control group, the difference being significant(P<0.05). Conclusion: The injection of dexamethasone combined with Jianpi Tongqiao prescription has better curative effect than that of simple western medicine in treating CSOM, which can significantly alleviate patients' clinical symptoms,improve their thresholds of bone conduction and levels of AQP-1 and AQP- 4 in serum,as well as reduce clinical recurrence rate.