益气固肾方对STZ 诱导的糖尿病肾病大鼠肾功能及 miR-192 的影响
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R587.2

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浙江省中医药科学研究基金项目(2017ZA111)


Effect of Yiqi Gushen Prescription on Kidney Function and miR- 192 in Rats with Dia⁃ betic Nephropathy Induced by STZ
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    摘要:

    目的:探讨益气固肾方对链脲佐菌素(STZ) 诱导的糖尿病肾病(DN) 大鼠肾功能及miR-192的影响,探讨其对DN的防治作用及作用机制。方法:将36 只SD 大鼠随机分为正常组、模型组、厄贝沙坦组、益气固肾方低、中、高剂量组(低、中、高剂量组),每组6只。除正常组外,复制DN大鼠模型。低、中、高剂量组分别按0.047 g/kg,0.141 g/kg,0.282 g/kg的剂量给予益气固肾方水煎液,厄贝沙坦组按20 mg/kg 的剂量给予厄贝沙坦,模型组和正常组大鼠灌服等容积生理盐水,每天灌胃1 次,持续6 周。检测各组大鼠摄食量、饮水量、尿量,采用全自动生化仪检测大鼠血清谷草转氨酶(ALT)、谷丙转氨酶(AST)、血尿素氮(BUN)、血肌酐(SCr),RT-PCR法定量检测miR-192 mRNA的表达。结果:与正常组比较,模型组大鼠摄食量、饮水量、尿量、血清ALT、AST、BUN、SCr 含量及miR-192 mRNA 表达均升高(P<0.05)。与模型组比较,厄贝沙坦组和中、高剂量组大鼠饮水量及尿量减少(P<0.05),低剂量组尿量减少(P<0.05);厄贝沙坦组和高剂量组ALT、AST、BUN、SCr含量均降低(P<0.05),中、低剂量组血清ALT、AST、BUN、SCr含量均降低,但仅ALT差异有统计学意义(P<0.05);厄贝沙坦组及益气固肾方各剂量组miR-192 mRNA 表达减少(P<0.05)。与厄贝沙坦组比较,中、低剂量组血清ALT、AST、SCr 含量较高(P<0.05);高剂量组miR-192 mRNA表达降低(P<0.05)。结论:益气固肾方可有效治疗DN,其机制可能与抑制DN大鼠肾脏miR-192的表达有关。

    Abstract:

    Abstract: Objective: To discuss the effect of Yiqi Gushen prescription on kidney function and miR- 192 in rats with diabetic nephropathy(DN) induced by streptozotocin(STZ),and its preventive and therapeutic effect and mechanism on DN. Methods: A total of 36 cases of SD rats were randomly divided into the normal group, the model group, the irbesartan group, and the groups of Yiqi Gushen prescription in low, middle and high dose(the low- dose group, the middle- dose group and the high-dose group),6 rats in each group. Except the normal group,the rat models with DN were replicated. The groups of Yiqi Gushen prescription in low,middle and high dose were given water decoction of Yiqi Gushen prescription in 0.047 g/kg,0.141 g/kg and 0.282 g/kg respectively. The irbesartan group was given irbesartan in 20 mg/kg. The model group and the normal group were administered with the equivalent volume of saline by gavage,once a day,for six weeks. The intragastric administration, drinking amount and urine volume of rats in each group were detected; automatic biochemical analyzer was used to detect alanine aminatransferase(ALT),aspartate aminotransferase(AST),blood urea nitrogen(BUN) and serum creatinine(SCr) ; RT-PCR method was used to quantitatively detect the expression of miR-192 mRNA. Results : Compared with those in the normal group,in the model group,the amount of food intake,drinking amount,urine volume, the contents of ALT,AST,BUN and SCr in serum,as well as the expression of miR-192 mRNA of rats were all increased (P<0.05). Compared with those in the model group, in the irbesartan group, the middle- dose group and the high- dose group,the drinking amount and urine volume of rats were decreased(P<0.05),and urine volume in the low-dose group was decreased(P<0.05);in the irbesartan group and the high-dose group,the contents of ALT,AST,BUN and SCr in serum were decreased(P<0.05),and the above contents in the low-dose group and the middle-dose group were decreased,(P< 0.05),but there was significant difference only being found in the comparison of ALT(P<0.05);in the irbesartan group and the groups of Yiqi Gushen prescription in each dose,the expression of miR-192 mRNA was decreased(P<0.05). Compared with those in the irbesartan group,in the low- dose group and the middle- dose group,the contents of ALT,AST and SCr in serum were higher(P<0.05);the expression of miR-192 mRNA in the high-dose group was decreased(P<0.05). Conclusion: Yiqi Gushen prescription can effectively treat DN, whose mechanism may be related to the inhibition of the expression of miR-192 in the kidney of rats with DN.

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孙炜,王云卿.益气固肾方对STZ 诱导的糖尿病肾病大鼠肾功能及 miR-192 的影响[J].新中医,2020,52(13):1-3

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  • 在线发布日期: 2020-07-10
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