蒲公英提取物调控DPC4对大肠癌细胞凋亡、迁移、 侵袭的影响及其机制研究
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

R273

基金项目:


Study on Effect of Herba Taraxaci Extract Regulating DPC4 on Apoptosis,Migration and Invasion of Colorectal Cancer Cells and Its Mechanism
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探讨蒲公英提取物通过调控胰腺癌缺失位点4(DPC4) 表达对大肠癌细胞凋亡、迁移和侵袭的影响和机制。方法:用不同终浓度(1.5、3.0、7.5、15.0 mg/mL) 的蒲公英提取物处理HT-29 细胞48 h 后,流式细胞术检测细胞凋亡,Transwell法检测细胞的迁移和侵袭能力,Western blot检测DPC4蛋白的表达。将pcDNA3.1-DPC4转染HT-29细胞,观察过表达DPC4对HT-29细胞凋亡、迁移和侵袭的影响。将si-DPC4转染HT-29细胞,给予蒲公英提取物处理后,观察抑制DPC4表达对HT-29细胞凋亡、迁移和侵袭的影响。结果:蒲公英提取物在体外可诱导HT-29细胞凋亡,抑制HT-29细胞的迁移和侵袭,并呈明显的浓度依赖性。15.0 mg/mL 的蒲公英提取物可促进DPC4 蛋白的表达。过表达DPC4 可显著促进HT-29 细胞凋亡,抑制HT-29细胞的迁移和侵袭。抑制DPC4表达能逆转蒲公英提取物对HT-29细胞凋亡、迁移和侵袭的影响。结论:蒲公英提取物通过上调DPC4表达进而促进大肠癌细胞凋亡,抑制细胞的迁移和侵袭能力。

    Abstract:

    Abstract: objective: To investigate the effect and mechanism of Herba Taraxaci extract on apoptosis, migration and invasion of colorectal cancer cells by regulating the expression of deleted in pancreatic carcinoma locus 4(DPC4). Methods: After treating HT-29 cells with Herba Taraxaci extract of different final concentrations including 1.5,3.0,7.5 and 15.0 mg/mL for 48 hours,cell apoptosis was detected by flow cytometry,the ability of migration and invasion of cells was detected by Transwell method,and expression of DPC4 protein was detected by Western blot. PcDNA3.1- DPC4 was transfected into HT-29 cells,and the effect of over-expression of DPC4 on apoptosis,migration and invasion of HT-29 cells was observed. Si-DPC4 was transfected into HT-29 cells,and the effect of the inhibition of DPC4 expression on apoptosis,migration and invasion of HT-29 cells was observed after being treated with Herba Taraxaci extract. Results:Herba Taraxaci extract in vitro could induce the apoptosis of HT-29 cells and inhibit the migration and invasion of HT-29 cells in an obvious concentrationdependent manner. Fifteen mg/mL of Herba Taraxaci extract can promote the expression of DPC4 protein. Over-expression of DPC4 could significantly promote the apoptosis of HT- 29 cells and inhibit the migration and invasion of HT- 29 cells. Inhibition of DPC4 expression could reverse the effect of Herba Taraxaci extract on apoptosis,migration and invasion of HT- 29 cells. Conclusion: By up- regulating the expression of DPC4, Herba Taraxaci extract can promote the apoptosis of colorectal cancer cells and inhibit the ability of migration and invasion of cells.

    参考文献
    相似文献
    引证文献
引用本文

杨庆华,陈栋.蒲公英提取物调控DPC4对大肠癌细胞凋亡、迁移、 侵袭的影响及其机制研究[J].新中医,2020,52(8):1-5

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2020-04-25
  • 出版日期: