| 摘要: |
| 目的：鉴定黄芪生脉饮中的主要天然糖类物质，并对其含量进行测定。方法：通过和标准品在带蒸发光检测器高效液相色谱（HPLC-ELSD） 上出峰时间的比较，来鉴定黄芪生脉饮中的主要天然糖类成分；采用COSMOSIL 5NH2-MS 氨基柱结合HPLC-ELSD 进行单糖含量测定，流动相为乙腈-水（80∶20），流速为1 mL/min，柱温35 ℃。结果：黄芪生脉饮中的主要天然糖类成分为果糖；基于氨基柱建立的HPLC-ELSD 果糖检测方法在0.2～16 mg/mL 的浓度范围内线性关系良好，平均回收率为98.63%，RSD 为1.76%。结论：黄芪生脉饮中含有较高的天然果糖成分，其含量可用HPLC-ELSD 法进行检测。 |
| 关键词: 黄芪生脉饮 果糖 HPLC-ELSD 法 含量测定 |
| DOI： |
|
| 基金项目:浙江省医药卫生科技项目（2017KY498） |
|
| Identification and Content Determination of Natural Fructose in Huangqi Shengmai Yin |
| LIN Yan,DAI Binling,LIN Changxin,WANG Jiaqi,CHEN Baichen,QIAN Chaodong |
| (单篇PDF全文查阅请上中国知网下载阅读（中国知网电子版刊出时间：纸刊刊出日期一个月左右）) |
| Abstract: |
| Abstract： Objective： To identify the main natural saccharides in Huangqi Shengmai yin， and to determinate their
contents. Methods： The main natural saccharides in Huangqi Shengmai yin were identified by comparing the peak time on
high-performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD) with that of standard reference
substance. The content determination of monosaccharide was performed by COSMOSIL 5NH2-MS amino column combined
with HPLC-ELSD；the mobile phase was acetonitrile-water(80：20)；the flow rate was 1 mL/min；the column temperature
was 35 ℃. Results：The main natural saccharide in Huangqi Shengmai yin was fructose. The HPLC-ELSD fructose detection
method based on amino column showed good linear relationship in the concentration range of 0.2 to 16 mg/mL， with an
average recovery rate of 98.63% and RSD of 1.76% . Conclusion： Huangqi Shengmai yin has relatively high content of
fructose which can be detected via HPLC-ELSD method. |
| Key words: Keywords：Huangqi Shengmai Yin Fructose HPLC-ELSD method Content determination |
