| 摘要: |
| 目的：探讨白芍提取物对白血病细胞增殖、凋亡的影响及其作用机制。方法：选用白血病细胞K652 为研究对象，分为对照组、白芍提取物不同浓度（20 μg/mL、30 μg/mL、40 μg/mL） 组、miR-NC 组、miR-564 组、白芍提取物30 μg/mL+anti-miR-NC 组及白芍提取物30 μg/mL+anti-miR-564 组。采用MTT 法检测各组细胞活性，流式细胞术检测各组细胞凋亡情况，实时荧光定量PCR 检测细胞中miR-564 表达，蛋白质印迹法检测各组细胞中细胞周期蛋白D1（CyclinD1）、细胞周期依赖性蛋白激酶抑制因子1A（P21）、B 淋巴细胞瘤-2（Bcl-2） 及B 淋巴细胞瘤-2 相关X 蛋白（Bax） 的表达。结果：与对照组比较，白芍提取物不同浓度组K652 细胞活性、Cyclin D1 均降低（P＜0.05），P21 表达水平升高（P＜0.05）；白芍提取物30 μg/mL 组Bcl-2蛋白表达水平降低（P＜0.05），细胞凋亡率、Bax 蛋白及miR-564 表达水平升高（P＜0.05）。与miR-NC 组比较，miR-564 组K652 细胞活性、Cyclin D1 及Bcl-2 蛋白表达水平均降低（P＜0.05），细胞凋亡率、P21、Bax 蛋白及miR-564 表达水平均升高（P＜0.05）。与白芍提取物30 μg/mL+anti-miR-NC 组比较，白芍提取物30 μg/mL+anti-miR-564 组K652 细胞活性、Cyclin D1及Bcl-2 蛋白表达水平均升高（P＜0.05），细胞凋亡率、P21、Bax 蛋白及miR-564 表达水平均降低（P＜0.05）。白芍提取物30 μg/mL+anti-miR-NC 组各检测指标与白芍提取物组30 μg/mL 组比较，差异无统计学意义（P＞0.05）。结论：白芍提取物可能通过上调miR-564 表达抑制白血病细胞增殖，并诱导细胞凋亡。 |
| 关键词: 白血病 白芍提取物 miR-564 增殖 凋亡 细胞实验 |
| DOI： |
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| 基金项目: |
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| Study on Effect of Extract of Radix Paeoniae Alba Regulating miR- 564 Expression onProliferation and Apoptosis of Leukemia Cells |
| WANG Dapeng,YANG Jian,LIU Xianhui |
| (单篇PDF全文查阅请上中国知网下载阅读（中国知网电子版刊出时间：纸刊刊出日期一个月左右）) |
| Abstract: |
| Abstract：Objective：To discuss the effect of extract of Radix Paeoniae Alba on proliferation and apoptosis of leukemia
cells and its mechanism. Methods： Leukemia cells K652 were selected as the study objects， and were divided into the
control group，the extract of Radix Paeoniae Alba groups of different concentrations，namely，20 μg/mL，30 μg/mL and
40 μg/mL，the miR-NC group，the miR-564 group，the group of 30 μg/mL extract of Radix Paeoniae Alba combined with
anti- miR- NC， and the group of 30 μg/mL extract of Radix Paeoniae Alba combined with anti- miR- 564. In each group，
activity was detected by MTT method；apoptosis was detected by flow cytometry；the real-time fluorescence quantitative
PCR was applied to detect miR- 564 expression. In each group， the expression of cyclinD1， cyclin- dependent kinase
inhibitor 1A(P21)， B cell lymphoma- 2(Bcl- 2)， and Bcl- 2 associated X protein(Bax) were detected by Western blotting.
Results：Compared with those in the control group，K652 cell activity，and the expression levels of CyclinD1 in the extract of
Radix Paeoniae Alba groups of different concentrations were decreased(P＜0.05)；the expression levels of P21 was increased
(P＜0.05)；Bcl-2 protein in the 30 μg/mL extract of Radix Paeoniae Alba group were decreased(P＜0.05)，and the apoptosis
rate，Bax protein and miR-564 were increased(P＜0.05). Compared with those in the miR-NC group，K652 cell activity，and
the expression levels of CyclinD1 and Bcl- 2 protein in the miR- 564 group were decreased(P＜0.05)； the apoptosis rate，
and the expression levels of P21，Bax protein and miR-564 were increased(P＜0.05). Compared with those in the group of
30 μg/mL extract of Radix Paeoniae Alba combined with anti- miR- NC， K652 cell activity， and the expression levels of
CyclinD1 and Bcl- 2 protein in the group of 30 μg/mL extract of Radix Paeoniae Alba combined with anti- miR- 564 were
increased(P＜0.05)；the apoptosis rate，and the expression levels of P21，Bax protein and miR- 564 were decreased(P＜
0.05). There was no significant difference being found in the comparison of each detection index between the group of 30 μg/
mL extract of Radix Paeoniae Alba combined with anti-miR-NC and the 30 μg/mL extract of Radix Paeoniae Alba group(P＞
0.05). Conclusion：The extract of Radix Paeoniae Alba probably inhibits proliferation of leukemia cells，and induce apoptosis
by up-regulating miR-564 expression. |
| Key words: Keywords：Leukemia Extract of Radix Paeoniae Alba miR-564 Proliferation Apoptosis Cell experiment |
