基于IRS-1信号通路探讨虎杖甙对2型糖尿病大鼠胰岛素抵抗的改善作用
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

R285.5

基金项目:

台州市科技计划项目(1902ky50)


Polydatin Has Effect on Improving Insulin Resistance in Rats with Type 2 Diabetes Based on IRS-1 Signaling Pathway
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探讨虎杖甙对2 型糖尿病大鼠胰岛素抵抗(IR) 的改善作用及其作用机制。方法:SPF 级SD 大鼠100 只,随机分为对照组,模型组,吡咯列酮组,虎杖甙低、高剂量组5 组,每组20 只。除对照组外,其余各组大鼠腹腔注射链脲佐菌素建立2 型糖尿病大鼠模型。吡咯列酮组,虎杖甙低、高剂量组于模型制备成功后第1 天开始灌胃相应药物,对照组和模型组灌胃等体积生理盐水。试验结束后,测定IR 相关指标,RT-PCR 法及Western blot 法测定胰腺胰岛素受体底物-1(IRS-1)、葡萄糖转运蛋白-4(GLUT-4) 基因和蛋白水平。结果:与对照组比较,模型组空腹血糖(FBG)、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR) 水平升高(P<0.05),胰岛β 细胞功能指数(HOMA-β)、胰岛素敏感性检测指数(QUICKI) 水平、胰腺IRS-1、GLUT-4 mRNA 及蛋白水平降低(P<0.05)。与模型组比较,吡咯列酮组、虎杖甙低、高剂量组FBG、FINS、HOMA-IR 水平降低(P<0.05),HOMA-β、QUICKI 水平、胰腺IRS-1、GLUT-4 mRNA 及蛋白水平升高(P<0.05)。与吡咯列酮组比较,虎杖甙低、高剂量组FBG、FINS、HOMA-IR 水平升高(P<0.05),HOMA-β、QUICKI 水平、胰腺IRS-1、GLUT-4 mRNA 及蛋白水平降低(P<0.05)。与虎杖甙低剂量组比较,虎杖甙高量组FBG、FINS、HOMA-IR 水平降低(P<0.05),HOMA-β、QUICKI 水平、胰腺IRS-1、GLUT-4 mRNA 及蛋白水平升高(P<0.05)。结论:虎杖甙能明显改善2 型糖尿病大鼠IR 作用,其机制可能与虎杖甙能促进2 型糖尿病大鼠胰腺IRS-1、GLUT-4 mRNA 及蛋白表达,进而激活IRS-1 信号通路有关。

    Abstract:

    Abstract:Objective:To discuss improvement effect of polydatin on insulin resistance(IR) in rats with type 2 diabetes and its mechanism. Methods: A total of 100 SD rates at SPF level were randomly divided into the control group, the model group, the pioglitazone group, and the polydatin groups of low and high dose, 20 rats in each group. Except the control group, the rat models with type 2 diabetes were established through intraperitoneal injection of streptozotocin in other groups. On the 1st day after successful establishment,the pioglitazone group and the polydatin groups of low and high dose were given gastric perfusion with corresponding medicine;the control group and the model group were given normal saline of same volume. After experiment,the relevant indexes of IR were determined;the levels of mRNA and protein of insulin receptor substrate-1(IRS-1) in the pancreas and glucose transporter-4(GLUT-4) were determined by RT-PCR method and Western blot method. Results: Compared with those in the control group, the levels of fasting plasma glucose(FBG), fasting insulin(FINS) and homeostasis model assessment of insulin resistance(HOMA-IR) in the model group were increased (P<0.05);the levels of homeostatic model assessment of islet β cell function(HOMA- β) and quantitative insulin sensitivity check index(QUICKI),and the levels of mRNA and protein of IRS- 1 in the pancreas and GLUT-4 were decreased(P<0.05). Compared with those in the model group,the levels of FBG,FINS and HOMA-IR in the pioglitazone group and the polydatin groups of low and high dose were decreased(P<0.05);the levels of HOMA- β and QUICKI,and the levels of mRNA and protein of IRS-1 in the pancreas and GLUT-4 were increased(P<0.05). Compared with those in the pioglitazone group,the levels of FBG, FINS and HOMA- IR in the polydatin groups of low and high dose were increased(P<0.05); the levels of HOMA-β and QUICKI,and the levels of mRNA and protein of IRS-1 in the pancreas and GLUT-4 were decreased(P<0.05). Compared with those in the low-dose polydatin group,the levels of FBG,FINS and HOMA-IR in the high-dose polydatin group were decreased(P<0.05);the levels of HOMA- β and QUICKI,and the levels of mRNA and protein of IRS- 1 in the pancreas and GLUT- 4 were increased(P<0.05). Conclusion: Polydatin can significantly improve IR in rats with type 2 diabetes. Its mechanism probably is that polydatin can promote the expression of mRNA and protein of IRS- 1 in the pancreas and GLUT-4,and then activate IRS-1 signaling pathway.

    参考文献
    相似文献
    引证文献
引用本文

应巧,何斐,张伟,泮瑛瑛,张霞霞.基于IRS-1信号通路探讨虎杖甙对2型糖尿病大鼠胰岛素抵抗的改善作用[J].新中医,2020,52(17):5-8

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2020-09-06
  • 出版日期: