Objective：To discuss the effect of aescin total saponins on uric acid reduction in vitro and in vivo and uric acid transporters of mice with hyperuricemia.Methods：Detected the inhibitory activity of aescin total saponins for xanthine oxidase in vitro.Divided 60 ICR mice into the normal control group，the model control group，the positive control group，the group of low-dose of aescin total saponins，the group of medium-dose aescin total saponins and the group of high-dose aescin total saponins randomly by vitro experiment.Adopted ethambutol and oteracil potassium to establish the model of mice with hyperuricemia.Allopurinol was used as a positive drug to respectively detect effects of aescin total saponins on serum uric acid level，hepatic xanthine oxidase activity and UrateTransporter1(URAT1)expression of kidney uric acid transporter protein of mice.Results：In vitro，aescin total saponins can inhibit activity of xanthine oxidase.In vivo experiment，comparing with mice in the model control group，serum uric acid levels and hepatic xanthine oxidase activity of mice in the group of low-dose of aescin total saponins，the group of medium-dose of aescin total saponins，and the group of high-dose of aescin total saponins were all decreased，and URAT1 expression of kidney uric acid transport protein was decreased，which were all dose-dependent to some degree.Conclusion：By inhibiting the activity of xanthine oxidase and reducing the expression of renal uric acid transporter URAT1，aescin total saponin can play a role in reducing uric acid，which has broad development prospects as a Chinese medicine of reducing uric acid.