Objective： To observe the effect of Yunpi granules on gastrointestinal motility and the expression of gastrointestinal hormone of rats with functional dyspepsia(FD)of liver-depression and spleen-deficiency type，and to discuss its functioning mechanism of preventing and treating FD.Methods：Divided 60 SD rats into the blank group，the model group，the low-dose，medium-dose and high-dose Yunpi granules groups and the domperidone group randomly，10 rats in each group，and applied combined factors mainly with moderate tail-clamping stimulation to establish the model of FD rats of liver-depression and spleen-deficiency type.Recorded the macroscopic token of rats in each group during experiment，measured the gastric emptying rate and the intestinal propulsive rate of rats in each group，and detected the expression level of substance P(SP)and vasoactive intestinal peptide(VIP)mRNA in gastrointestinal tissue of rats in each group by means of RT-PCR.Results：Comparing with the blank group，the gastric emptying rate and the intestinal propulsive rate of rats in the model group were both decreased to different degrees，the expression levels of SP mRNA in colon tissue were decreased，and the expression levels of VIP mRNA in gastric antrum tissue were increased，differences being significant(P＜0.01).Comparing with the model group，the gastric emptying rate and the intestinal propulsive rate of rats in the groups of Yunpi granules in each dose and the domperidone group were significantly increased，the expression levels of SP mRNA in colon tissue were increased，and the expression levels of VIP mRNA in gastric antrum tissue were decreased；the expression levels of SP mRNA in gastric antrum tissue of rats in the medium-dose and high-dose Yunpi granules group were increased，and the expression levels of VIP mRNA in colon tissue were decreased，differences being significant(P＜0.05，P＜0.01).Comparing with the domperidone group，the expression levels of SP mRNA in colon and gastric antrum tissue of rats in the medium-dose and high-dose spleen-activating granules group were increased，and the expression levels of VIP mRNA were decreased，differences being significant(P＜0.05).Conclusion：By multi-target birectional regulation of the levels of gastrointestinal hormone SP and VIP mRNA in FD rats，Yunpi granules promote gastric emptying and intestinal propulsive function of FD rats，and further improve gastrointestinal motility and paresthesia of FD rats，which is one of the functioning mechanisms of Yunpi granules for preventing and treating FD.