姜黄素预处理对肺缺血再灌注损伤大鼠的影响
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R56

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广东省中医药局项目(20152146)


Curcumin Pretreatment Has Effect on Rats with Lung Ischemia Reperfusion Injury
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    摘要:

    目的:探讨姜黄素预处理对肺缺血再灌注损伤大鼠的影响。方法:健康成年雄性SD大鼠48只,随机分为假手术组(S组)、缺血再灌注组(IR组)、姜黄素低剂量组(C50组) 及姜黄素高剂量组(C200组),每组12只。C50组和C200组分别于麻醉前2 h腹腔一次性注射姜黄素50 mg/kg和200 mg/kg,行夹闭左肺门60 min再灌注180 min。S组于麻醉前2 h腹腔注射等容量生理盐水,开胸后左肺门不进行夹闭,旷置240 min。IR组于麻醉前2 h腹腔注射等容量生理盐水,左肺门夹闭,左肺不张60 min再灌注180 min。于缺血前、再灌注60、120、180 min时采集右颈动脉血行血气分析,并计算呼吸指数(RI)。再灌注末处死动物,取肺组织,观察肺组织病理学变化并行肺损伤评分,测定肺湿干重比(W/D),检测超氧化物歧化物(SOD)和丙二醛(MDA)的含量。结果:与缺血前比较,IR组再灌注各时间点RI值显著升高(P<0.05)。与S组比较,IR组再灌注各时间点RI值显著升高,MDA含量、W/D和肺损伤评分升高,SOD活性降低,差异均有统计学意义(P<0.05)。与IR组比较,C50组和C200组再灌注各时间点RI值显著降低,MDA含量、W/D和肺损伤评分降低,SOD活性升高,差异均有统计学意义(P<0.05)。与C50组比较,C200组再灌注各时间点RI值显著降低,MDA含量、W/D和肺损伤评分降低,SOD活性升高,差异均有统计学意义(P<0.05)。结论:姜黄素预处理可减轻肺缺血再灌注损伤,且呈剂量依赖性,其机制可能与姜黄素减轻肺水肿,减少氧化产物的生成和增强清除氧自由基的能力有关。

    Abstract:

    Objective:To discuss the effect of curcumin pretreatment on rats with lung ischemia reperfusion injury.Methods:Divided 48 healthy adult male SD rats into sham operation group(group S),ischemia reperfusion group(group IR),low-concentration curcumin group(group C50)and high-concentration group(group C200)randomly,12 rats in each group.Group C50 and group C200 receiveddisposablel abdominal injection of curcumin(50 mg/kg and 200mg/kg respectively)2 h before anesthesia,occluding left pulmonary hilus for 60 min and reperfusing for 180 min.Group S received abdominal injection of equivalent saline 2 h before anesthesia,no occlusion of the left pulmonary hilus after opening chest,and was put aside for 240 min.Group IR received abdominal injection of equivalent saline 2 h before anesthesia,occluding leftpulmonary hilus for 60 min and reperfusing for 180 min.Before ischemia and in the 60th,120th and 180th min of reperfusion,collected the blood of right carotid artery to conduct a blood gas analysis,and calculated the respiratory index(RI).In the end of reperfusion,the rats were sacrificed for collecting the pulmonary tissue.Observed the pathological changes of the pulmonary tissue,measured wet-dry weight ratio(W/D),detected the content of superoxide dismutase(SOD)and malondialdehyde(MDA).Results:Comparing with that before ischemia,RI of the group IR in each time point of reperfusion was significantly increased(P< 0.05).Comparing with that of group S,RI of the group IR in each time point of reperfusion was significantly increased,the content of MDA,W/D and scores of pulmonary injury were higher,while the activation of SOD was lower,differences being significant(P< 0.05).Comparing with that of group IR,RI of the group C50 and group C200 in each time point of reperfusion wassignificantly decreased,the content of MDA,W/D and scores of pulmonary injury were lower,while the activation of SOD washigher,differences being significant(P< 0.05).Comparing with that of group C50,RI of the group C200 in each time point of reperfusion was significantly decreased,the concentration of MDA,W/D and scores of pulmonary injury were lower,while the activation of SOD was higher,differences being significant(P< 0.05).Conclusion:Curcumin pretreatment can release lung ischemia reperfusion injury in a dose-dependent manner,and the mechanism may be relevant with its curcumin of releasing pulmonary edema,reducing the formation of oxidation products and enhancing the function of clearing oxygen free radicals.

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吴建魏,刘洁,谭利斌,李向宇,叶飞,何强.姜黄素预处理对肺缺血再灌注损伤大鼠的影响[J].新中医,2018,50(1):7-10

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  • 在线发布日期: 2018-05-23
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