| 摘要: |
| 目的:观察通腑泻热方对脓毒症大鼠肺功能的保护作用。方法:选用SPF级SD大鼠120只,随机分为6组,每组20只,分别为空白对照组、假手术组、模型组、中药低剂量组、中药中剂量组、中药高剂量组。除空白对照组外,假手术组仅开腹,取出盲肠、放回腹腔、关腹,其他各组行盲肠结扎穿孔术制作脓毒症动物模型。中药低、中、高剂量组分别于造模后6h、18h、30h、42h给予通腑泻热方4.5g/（kg·d）、9g/（kg·d）、18g/（kg·d）灌胃,模型组、空白对照组、假手术组按同样时点给予相应量生理盐水灌胃。观察48h后大鼠生存率,经腹主动脉取大鼠动脉血进行血气分析及检测血清内毒素和降钙素原。结果:假手术组与空白对照组比较,各个指标均无统计学差异（P>0.05）,说明单纯手术不影响各个指标的检测结果。与假手术组比较,模型组和中药高、中、低各剂量组大鼠的生存率显著降低,差异均有统计学意义（P<0.01）。与空白对照组比较,模型组大鼠内毒素与降钙素原水平显著升高,大鼠动脉血PaO2、pH显著降低,PaCO2显著升高,差异均有统计学意义（P<0.05）,说明模型成立。与模型组比较,中药高、中、低各剂量组大鼠内毒素与降钙素原水平显著降低,大鼠动脉血PaO2、pH显著升高,PaCO2显著降低,差异均有统计学意义（P<0.05）。与中药低剂量组比较,中药高、中剂量组大鼠内毒素与降钙素原水平显著降低,大鼠动脉血PaO2、pH显著升高,PaCO2显著降低,差异均有统计学意义（P<0.05）。结论:通腑泻热方能够降低脓毒症大鼠血清降钙素原及内毒素水平,改善因脓毒症导致的急性肺损伤时大鼠的氧和指标,从而对脓毒症导致的大鼠急性肺损伤的肺功能有保护作用。 |
| 关键词: 脓毒症 通腑泻热方 肺功能 降钙素原 内毒素 动物模型 大鼠 |
| DOI： |
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| 基金项目:广东省自然基金；国家中医药管理局中医药科学技术研究专项课题 |
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| Abstract: |
| Abstract：Objective：To observe the protective effect of Tongfu Xiere prescription on pulmonary function of rats with sepsis.Methods：Selected 120 SPF SD rats，and divided them into six groups，namely the blank control group，the sham operation group，the model group，Chinese medicine low dosage group，Chinese medicine middle dosage group，and Chinese medicine high dosage group，20 cases in each.Except the normal control group，cecum of rats in the sham operation group were taken out and put back to abdominal cavity，and other groups all receivedcecal ligation and puncture to establish the animal model of sepsis.When the model was established after 6 h，18 h，30 h，and 42 h，Chinese medicine low，middle and high dosage groups were respectively given Tongfu Xiere prescription of 4.5 g/(kg·d)，9 g/(kg·d)and 18 g/(kg·d)by gavage.The model group，the sham operation group and the blank control group were respectively given the normal saline with corresponding dosage at the above time points.Observed the survival rate of rats after 48 h，conducted blood gas analysis of arterial blood in the abdominal aorta of rats，and detected serum endotoxin as well as procalcitonin.Results：No significant difference was found in the comparison of all indexes in the sham operation group and blank control group(P＞0.05)，which suggested that pure operation had no effect on all the indexes.Comparing with that in the sham operation group，the survival rate of rats in the model group and Chinese medicine low，middle and high dosage groups were significantly decreased，differences being significant(P＜0.01).Comparing with those in the blank control group，in the model group，levels of serum endotoxin and procalcitonin were significantly increased，while PaO2and pH in arterial blood were obviously declined，and PaCO2was markedly risen，difference being significant(P＜0.05)，implying the model was valid.Comparing with those in the model group，in Chinese medicine low，middle and high dosage groups，levels of serum endotoxin and procalcitonin were significantly decreased，while PaO2and pH in arterial blood were obviously increased，and PaCO2was markedly reduced，difference being significant(P＜0.05).Comparing with that in Chinese medicine low dosage group，in Chinese medicine middle and high dosage groups，levels of serum endotoxin and procalcitonin were significantly decreased，while PaO2and pH in arterial blood were obviously increased，and PaCO2was markedly reduced，difference being significant(P＜0.05).Conclusion：Tongfu Xiere prescription can reduce the levels of serum endotoxin and procalcitonin in rats with sepsis and improve oxygenation indexesin rats，so as to exert the protective effect on the pulmonary function of acute lung injury caused by sepsis. |
| Key words: Sepsis Tongfu Xiere prescription Pulmonary function Procalcitonin Endotoxin Animal model Rats |
