| 摘要: |
| 目的：探讨黄连素（Ber） 对树突状细胞（DC） 成熟表型和吞噬功能的影响。方法：用Ber 低、中、高浓度处理DC后，用荧光素标记单抗CD11c 和异硫氰酸荧光素（FITC） 标记单抗主要组织相容性复合体Ⅰ类（MHC Ⅰ）、主要组织相容性复合体II 类（MHC Ⅱ）、CD40、CD54、CD80、CD86、白细胞介素-12 （IL-12）、肿瘤坏死因子-α （TNF-α） 以及右旋糖酐（dextran） 双标DC，用流式细胞仪检测其表型、细胞因子的表达及吞噬能力的变化。结果：与对照组比较，Ber 各浓度组MHC Ⅰ、MHC Ⅱ、CD54、CD80、CD86 的表达均升高（P＜0.05），CD40 表达降低（P＜0.05）；与低浓度组比较，中、高浓度组MHC Ⅰ、MHC Ⅱ、CD54、CD86 的表达均增加（P＜0.05），CD40 表达下降（P＜0.05）；与中浓度组比较，高浓度组MHC Ⅰ、MHC Ⅱ、CD54、CD80、CD86 的表达均显著增加（P＜0.05），而CD40 表达下降（P＜0.05）。与对照组比较，Ber 各浓度组胞内细胞因子IL-12、TNF-α 的表达水平均升高（P＜0.05），且随Ber 浓度的增加而增加（P＜0.05）；中、高浓度组DC 的吞噬活性降低（P＜0.05）。结论：Ber 能够改变DC 的成熟表型和吞噬功能，提示Ber 可能通过DC 影响机体的免疫应答。 |
| 关键词: 树突状细胞 黄连素 表型 细胞因子 吞噬 细胞实验 动物实验 小鼠 |
| DOI： |
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| 基金项目:广东省自然科学基金项目（2017A030310666）；广东医科大学南海海洋生物医药资源研发公共服务平台开放式基金项目（2HC18013） |
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| Study on Berberine Regulating Phenotype and Phagocytosis of Dendritic Cells |
| CHEN Zhi,WANG Dongming,YE Xiaomei,YUAN Kangrui,CAI Kangrong,LIU Xiaoliu,WU Dudu |
| (单篇PDF全文查阅请上中国知网下载阅读（中国知网电子版刊出时间：纸刊刊出日期一个月左右）) |
| Abstract: |
| Abstract： Objective： To discuss the effect of berberine(Ber) on mature phenotype and phagocytosis of dendritic cells
(DC). Methods：DCs were treated with Ber at low，medium and high concentration respectively. After that，fluorescein was
used to mark monoclonal antibody CD11c； fluorescein isothiocyanate(FITC)， a kind of fluorescein was used to mark
monoclonal antibody major histocompatibility complex Class Ⅰ(MHC Ⅰ)，major histocompatibility complex Class Ⅱ(MHC Ⅱ)，
CD40，CD54，CD80，CD86，interleukin-12(IL-12) and tumor necrosis factor-α(TNF-α)；dextran was used to double label
DC. Flow cytometry was applied to detect the phenotype，the expression of cytokine and changes in phagocytosis. Results：
Compared with that in the control group，the expression of MHC Ⅰ，MHC Ⅱ，CD54，CD80 and CD86 in groups of Ber at
various concentrations was increased(P＜0.05)，and the expression of CD40 was decreased(P＜0.05). Compared with that in
the low concentration group，in the medium and high concentration groups，the expression of MHC Ⅰ，MHC Ⅱ，CD54 and
CD86 was increased(P＜0.05)， and the expression of CD40 was decreased(P＜0.05). Compared with that in the medium
concentration group，in the high concentration group，the expression of MHC Ⅰ，MHC Ⅱ，CD54，CD80 and CD86 was
significantly increased(P＜0.05)， and the expression of CD40 was decreased(P＜0.05). Compared with that in the control
group，the expression level of intracellular cytokines including IL- 12 and TNF- α in groups of Ber at various concentrations
was increased(P＜0.05)，and the expression was increased with the increase of Ber concentration(P＜0.05). Compared with
that in the control group，in the medium and high concentration groups，the phagocytosis of DC was significantly decreased
(P＜0.05). Conclusion：Ber can change the mature phenotype and phagocytosis of DC，which indicates that Ber may affect immune response of the body through DC. |
| Key words: Keywords： Dendritic cells Berberine Phenotype Cytokines Phagocytosis Cell experiment Animal experiment Mice |
