人参皂苷Rg3调控LncRNA CDKN2B-AS1 对胃癌细胞抑制作用的研究
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R285.5

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宁波市科学技术局市级科技计划项目(20191JCGY010063)


Study on Inhibitory Effect of Ginsenoside Rg3 Regulating LncRNA CDKN2B- AS1 on Gastric Cancer Cells
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    摘要:

    目的:探讨人参皂苷Rg3 调控长链非编码RNA(LncRNA)CDKN2B-AS1 对胃癌细胞的抑制作用。方法:选用胃癌细胞系SGC-7901,在培养液中加入人参皂苷Rg3 的胃癌细胞确定为研究组,同时未加药物培养的胃癌细胞为对照组。培养72 h 后用RT-PCR 法检测细胞中LncRNA CDKN2B-AS1、神经钙粘蛋白(N-cadherin)、上皮钙粘蛋白(E-cadherin)、血管内皮生长因子(VEGF)、B 淋巴细胞瘤-2(Bcl-2) 与Bcl-2 相关X 蛋白(Bax) mRNA 表达水平;用免疫印迹法检测细胞中细胞外调节蛋白激酶1/2(ERK1/2)、磷酸化激活的ERK1/2(p-ERK1/2)、基质金属蛋白酶-9(MMP-9) 表达水平;用MTT 试剂盒检测2 组细胞的增殖抑制率;用CCK-8 试剂盒检测2 组细胞增殖水平。结果:与对照组比较,研究组细胞LncRNA CDKN2B-AS1、VEGF、N-cadherin、Bcl-2 mRNA 及ERK1/2、p-ERK1/2、MMP-9 表达降低(P<0.05);Bax 及E-cadherin mRNA 表达升高(P<0.05);细胞增长抑制率升高(P<0.05),细胞增殖能力下降(P<0.05)。结论:在胃癌细胞的培养过程中加入人参皂苷Rg3能够有效抑制癌细胞的增殖与上皮间充质转化(EMT),为进一步临床试验提供可靠的理论基础。

    Abstract:

    Abstract: Objective: To investigate the inhibitory effect of ginsenoside Rg3 regulating long- chain noncoding RNA (LncRNA) CDKN2B- AS1 on gastric cancer cells. Methods: The gastric cancer cell line SGC- 7901 was selected; the gastric cancer cells in the culture medium with ginsenoside Rg3 were identified as the study group,and the gastric cancer cells without adding drugs were identified as the control group. After 72 hours of culture,the expression levels of mRNA including LncRNA CDKN2B-AS1,neural cadherin(N-cadherin),epithelial cadherin(E-cadherin),vascular endothelial growth factor(VEGF),B cell lymphocytoma-2(Bcl-2) and Bcl-2 related X protein(Bax) in the cells were detected by RT-PCR method ; the expression levels of extracellular signal- regulated kinase(ERK1/2) , ERK1/2 activated by phosphorylation (p-ERK1/2) and matrix metalloproteinase-9(MMP-9) in the cells were detected by Western Blotting;the inhibition rates of cell proliferation in the two groups were detected with MTT kit;cell proliferation levels in the two groups were detected by CCK-8 kit. Results:Compared with those in the control group,the expressions of mRNA including LncRNA CDKN2B-AS1, VEGF,N-cadherin,Bcl-2 mRNA as well as ERK1/2,p-ERK1/2 and MMP-9 in the cells in the study group were decreased (P<0.05); the mRNA expressions of Bax and E- cadherin were increased(P<0.05); the inhibition rate of cell growth was increased(P<0.05);the ability of cell proliferation was decreased(P<0.05). Conclusion:During the culture of gastric cancer cells, adding ginsenoside Rg3 can effectively inhibit the proliferation of cancer cells and epithelial- mesenchymal transition (EMT),which provides a reliable theoretical basis for further clinical trials.

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楼亭,胡学谦,张婷素,袁春樱.人参皂苷Rg3调控LncRNA CDKN2B-AS1 对胃癌细胞抑制作用的研究[J].新中医,2020,52(17):1-4

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  • 在线发布日期: 2020-09-06
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