苦参素联合扁桃苷治疗小鼠肝纤维化的相关研究
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R285.5

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广州市属高校科技项目 (1201410075):广州市羊城学者项目 (1201561579)


Correlation Study on Matrine Combined with Amygdalin for Hepatic Fibrosis in Mice
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    摘要:

    目的:研究苦参素联合扁桃苷治疗肝纤维化的作用。方法:将40只雄性C57BL/6小鼠分为正常组5只、模型组5只、苦参素组10只、扁桃苷组10只、联合用药组10只。用四氯化碳(CCl4)诱导肝纤维化小鼠模型,天狼星红染色确定肝纤维化程度和纤维染色面积;通过试剂盒检测羟脯氨酸浓度,评价纤维沉积情况;检测血谷丙转氨酶(Alanine aminotransferase,ALT)和谷草转氨酶(Aspartate aminotransferase,AST)含量变化。结果:与正常组比较,模型组小鼠体质量明显减轻,血ALT与AST水平上升,肝纤维化明显,差异均有统计学意义(P<0.01);与模型组比较,苦参素组、扁桃苷组和联合用药组小鼠平均体质量均有所升高,但仅联合用药组差异有统计学意义(P<0.01);苦参素组、扁桃苷组和联合用药组小鼠肝组织肝纤维化Ishak评分、天狼星红胶原染色面积、羟脯氨酸占比及血ALT、AST水平均明显下降,以联合用药组效果最明显,差异均有统计学意义(P<0.05,P<0.01)。结论:苦参素与扁桃苷联合应用具有显著的抗肝纤维化作用,且治疗效果明显优于单用苦参素或扁桃苷。

    Abstract:

    Objective:To study the anti-hepatic fibrosis effect of matrine combined with amygdalin.Methods:Forty male C57BL/6 mice were divided into five groups,included five in the normal group,five in the model group,ten in the matrine group,ten in the mandelic group and ten in the combined treatment group.Established the model of mice with hepatic fibrosis by induction of carbon tetrachloride,and determined the degree of hepatic fibrosis and the staining area of fibrosis via Sirius red staining;evaluated the concentration of hydroxyproline and detected the status of fibrous deposition by means of kits;detected the changes of contents of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in serum.Results:Comparing with the normal group,the body mass of mice in the model group was significantly decreased,the levels of ALT and AST in serum were increased,and hepatic fibrosis was evident,differences being significant(P<0.01).Comparing with the model group,the average body mass of mice in the matrine group,the amygdalin group and the combination group was increased respectively,but there was significant difference being found only in the combination group(P<0.01).The Ishak scores of hepatic fibrosis in liver tissue of mice,the area of Sirius red collagen staining,concentration of hydroxyproline and levels of ALT and AST in serum were evidently decreased in all groups,among which the effect in the combination group was the most evident,differences being significant(P<0.05,P<0.01).Conclusion:The combined application of matrine and amygdalin has significant anti-hepatic fibrosis effect,and its therapeutic effect is evidently better than the simple application of matrine and amygdalin.

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闫冰川,江鹏,江伟炽,洪健.苦参素联合扁桃苷治疗小鼠肝纤维化的相关研究[J].新中医,2018,50(12):25-27

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  • 在线发布日期: 2018-12-09
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