清化固肾排毒颗粒对单侧输尿管梗阻大鼠肾组织TGF-β1和α一SMA表达的影响
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

R693

基金项目:

浙江省自然科学基金项目(LY14H270001);浙江省中医药科学研究基金计划项目(2013ZB121)


Qinghua Gushen Paidu Granules Has Effect on TGF-β1 and α-SMA Expression of Nephridial Tissue of Rat with Unilateral Ureteral Obstruction
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:观察清化固肾排毒颗粒对单侧输尿管梗阻大鼠肾组织转化生长因子-β1(TGF-β1) 和α-平滑肌肌动蛋白(α-SMA)表达的影响,探讨其抗肾纤维化的作用机制。方法:72只SPF级SD大鼠随机分为假手术组、模型组、洛丁新组及清化固肾排毒颗粒组,除假手术组外,其余各组大鼠均采用单侧输尿管结扎手术方法(UUO)建立肾纤维化梗阻大鼠模型,术后各组分别予相应药物或生理盐水灌胃,每天1次。第7、14、21天,各组大鼠禁食不禁水1天,处死,取肾组织做病理检测,HE染色观察其病理变化,免疫组织化学染色检测TGF-β1和α-SMA在肾间质的阳性染色表达。结果:与假手术组比较,干预第7、14、21天,模型组、洛丁新组、清化固肾排毒颗粒组大鼠肾小管间质损伤指数显著增加,肾组织TGF-β1和α-SMA的表达均明显升高,差异均有统计学意义(P<0.05)。与模型组比较,干预第7、14、21天,洛丁新组和清化固肾排毒颗粒组大鼠肾小管间质损伤指数明显减轻,肾组织TGF-β1和α-SMA的表达均明显降低,差异均有统计学意义(P<0.05)。结论:清化固肾排毒颗粒可能通过下调TGF-β1和α-SMA的表达来抑制上皮细胞间充质转化和细胞外基质的过度积聚,实现改善肾间质纤维化的作用。

    Abstract:

    Objective:To observe the effect of Qinghua Gushen Paidu granules on expression of transforming growth factor-β1(TGF-β1)and α-smooth muscle actin(α-SMA)of nephridial tissue of rats with unilateral ureteral obstruction,and investigate its mechanism of anti-renal fibrosis.Methods:Divided 72 SD rats of SPF grade into sham group,model group,Lotensin group,and Qinghua Gushen Paidu granules group randomly.In addition to the sham group,all groups were given unilateral ureteral occlusion(UUO)to establish rat models of renal fibrosis obstruction and respectively receivedmedication or saline by gavage after surgery,once a day.On the 7th,14th,and 21stday of treatment,rats in each group fasted for one day without water-fasting and were sacrificed.Then kidney tissue of the rats were collected for pathology test.Observed pathological changes by using HE staining, and detected positive staining expression of TGF- β1 and α-SMA in renal interstitium by immunohistochemical staining.Results:Compared with the sham group,on the 7th, 14th, and 21stday of intervention,the damage indexes of renal tubular interstitium in other groups were evidently improved,and the expression of TGF-β1 and α-SMA of kidney tissue was also obviously increased,differences being significant(P< 0.05).Compared with the model group, on the 7th, 14th, and 21stday of intervention, the damage indexes of renal tubular interstitium and theexpression of TGF- β1 and α-SMA of renal tissue in the Lotensin group and Qinghua Gushen Paidu granules group were evidently decreased,differences being significant(P< 0.05).Conclusion:Qinghua Gushen Paidu granules may prevent the mesenchymal transition in epithelial cells and excessive accumulation of matrix out of epithelial cells by reducing the expression of TGF- β1 and α-SMA so as to improve renal fibrosis.

    参考文献
    相似文献
    引证文献
引用本文

汪国敏,李丽燕,吴小燕,林文博,冯雨林,董飞侠.清化固肾排毒颗粒对单侧输尿管梗阻大鼠肾组织TGF-β1和α一SMA表达的影响[J].新中医,2018,50(1):1-6

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2018-01-05
  • 出版日期: